中国中医药信息杂志2024，Vol.31Issue(8)：23-28,6.DOI:10.19879/j.cnki.1005-5304.202306415
基于网络药理学及实验验证探讨除风益损汤治疗角膜损伤机制
Exploration on Machanism of Chufeng Yisun Decoction in the Treatment of Corneal Injury Based on Network Pharmacology and Experimental Validation
摘要
Abstract
Objective To study the mechanism of Chufeng Yisun Decoction in the treatment of corneal injury based on network pharmacology combined with experimental validation.Methods The active components and targets of Chufeng Yisun Decoction were obtained from TCMSP and TCMID databases.Related targets of corneal injury were searched through GeneCards,OMIM,TTD and NCBI-Gene databases.Chinese materia medica-active components-key target network was established.The main active components of Chufeng Yisun Decoction for the treatment of corneal injury were analyzed.The core targets were predicted through PPI network.CCK-8 method was used to screen the optimal concentration of serum containing Chufeng Yisun Decoction for promoting cell growth.Western blot was used to detect autophagy related protein expressions of LC3,LAMP1 and ERK2.Results The main active components of Chufeng Yisun Decoction in the treatment of corneal injury were kaempferol,wogonin,quercetin and paeoniflorin.The core targets were AKT1,TP53,MAPK1,JUN and TNF.The intervention of serum containing Chufeng Yisun Decoction on human corneal fibroblasts could increase the LC3I/LC3II ratio and LAMP1 protein expression,while decrease ERK2 protein expression,which was consistent with the prediction of network pharmacology.Conclusion Chufeng Yisun Decoction treats corneal injury through multiple components,targets and pathways.The mechanism of promoting autophagy therapy for corneal injury is achieved by down-regulating the expression of ERK2 and up-regulating the expression of LC3 and LAMP1.关键词
除风益损汤/角膜损伤/自噬/炎症/网络药理学Key words
Chufeng Yisun Decoction/corneal injury/autophagy/inflammation/network pharmacology分类
医药卫生引用本文复制引用
李江伟,陈惠媚,张文清,欧晨,陈雄,姚小磊,彭清华..基于网络药理学及实验验证探讨除风益损汤治疗角膜损伤机制[J].中国中医药信息杂志,2024,31(8):23-28,6.基金项目
国家自然科学基金(82174443、81860870) （82174443、81860870）
国家中医药管理局人才支持项目-岐黄学者(2022年) （2022年）
国家中医药管理局中医眼科学重点学科建设项目(ZK1801YK015) （ZK1801YK015）
湖南省自然科学基金(2021JJ30527) （2021JJ30527）
湖南省卫生健康高层次人才计划(2022年) （2022年）
湖南省教育厅科学研究项目(20A370) （20A370）
湖南省中医药科研计划项目(2021023) （2021023）
湖南省研究生科研创新项目(CX20220782) （CX20220782）
湖南中医药大学中医学一流学科开放基金(2021ZYX05) （2021ZYX05）
