中国中医药信息杂志2024，Vol.31Issue(8)：70-75,6.DOI:10.19879/j.cnki.1005-5304.202310601
基于p38MAPK信号通路探讨火针治疗膝骨关节炎作用机制
Discussion on the Mechanism of Fire Needle in Treating Knee Osteoarthritis Based on p38MAPK Signaling Pathway
摘要
Abstract
Objective To explore the mechanism of fire needle in treating cartilage degeneration in knee osteoarthritis(KOA)based on p38MAPK signaling pathway.Methods Totally 30 SPF grade male SD rats were randomly divided into blank group,model group and fire needle group,with 10 rats in each group.The model group and fire needle group established KOA models by injecting papain into the knee joint cavity.The fire needle group received fire needle intervention in"Neixiyan"and"Dubi",while the model group and blank group only received fixation and no intervention,once every 3 days,for 4 consecutive weeks.Morphological changes in the knee joint cartilage were observed using HE staining and safranine O-solid green staining,TUNEL staining was used to detect the apoptosis index of chondrocytes,immunohistochemical staining was used to detect the expressions of p-p38 and Fas protein,qPCR was used to detect the expressions of p38 and Fas mRNA.Results Compared with the blank group,the surface of the knee joint cartilage in the model group rats was uneven,with irregular and uneven arrangement of chondrocytes,lighter matrix red staining,and unclear tidal lines,the improved Mankin's score significantly increased(P<0.001),the apoptosis index of chondrocytes significantly increased(P<0.01),the expressions of p-p38 and Fas protein in knee joint cartilage tissue significantly increased(P<0.01),and the expressions of p38 and Fas mRNA significantly increased(P<0.001).Compared with the model group,the surface of the knee joint cartilage of the fire needle group rats was relatively flat,the arrangement of chondrocytes was generally normal,and the matrix staining was slightly lighter,the improved Mankin's score significantly decreased(P<0.001),the apoptosis index of chondrocytes significantly decreased(P<0.01),the expressions of p-p38 and Fas protein in knee joint cartilage tissue significantly decreased(P<0.01),and the expressions of p38 and Fas mRNA significantly decreased(P<0.001).Conclusion Fire needle may delay the progression of KOA by inhibiting the activation of p38MAPK signaling pathway and reducing chondrocyte apoptosis of knee joint.关键词
火针/膝骨性关节炎/软骨细胞/p38MAPK信号通路/凋亡Key words
fire needle/knee osteoarthritis/chondrocytes/p38MAPK signaling pathway/apoptosis分类
医药卫生引用本文复制引用
万甜,肖阳,石欣悦,洪昆达..基于p38MAPK信号通路探讨火针治疗膝骨关节炎作用机制[J].中国中医药信息杂志,2024,31(8):70-75,6.基金项目
福建省卫健委中青年骨干人才项目(2020GGA055) （2020GGA055）
福建省自然科学基金面上项目(2023J01732) （2023J01732）
