中国中医药信息杂志2024，Vol.31Issue(8)：76-83,8.DOI:10.19879/j.cnki.1005-5304.202310094
基于PPARγ/NF-κB信号通路探讨防己黄芪消肿方干预代谢综合征表型骨关节炎作用机制
Discussion on the Mechanism of Intervention of Fangji Huangqi Xiaozhong Prescription in Metabolic Syndrome Phenotype Osteoarthritis Based on PPARγ/NF-κB Signaling Pathway
摘要
Abstract
Objective To explore the treatment effects and mechanism of Fangji Huangqi Xiaozhong Prescription in metabolic syndrome phenotype osteoarthritis(MS-OA)based on PPARγ/NF-κB signaling pathway.Methods SD rats were randomly divided into sham-operation group,OA group,MS-OA group,Western medicine group,and TCM high-and low-dasage groups.The modified Hulth method was used to make the OA model,and OA model was added with high-carbohydrate high-fat diet to make the MS-OA model.TCM high-and low-dosage groups were given 15.12,7.56 g/kg Fangji Huangqi Xiaozhong Prescription for gavage.The Western medicine group was given 16.2 mg/kg of losoprofen sodium by gavage,while the other groups were given physiological saline by gavage once a day for 6 consecutive weeks.Rat body mass was measured,biochemical detection of blood lipids and blood glucose was conducted,ELISA was used to detect the contents of serum TNF-α,IL-1β,IL-10 and leptin,morphological changes in cartilage tissue were observed using safranin O-fixed green and HE staining,immunohistochemical staining was used to detect expressions of Acan,ColⅩ,MMP13,TNF-α,IL-1β and PPARγ in cartilage tissue,Western blot was used to detected the expression of PPARγ,NF-κBp65 and p-NF-κBp65 protein in cartilage tissue.Results Compared with the sham-operation group,body mass and serum TC,TG,LDL-C,TNF-α,IL-1β and leptin of MS-OA group increased significantly(P<0.01),the contents of HDL-C and IL-10 decreased(P<0.01),cartilage tissue degeneration was significant,and the Mankin score increased(P<0.01),the expression of ColⅩ,MMP13,TNF-α,IL-1β,p-NF-κBp65 protein increased(P<0.05,P<0.01),and the expression of Acan and PPARγ protein decreased(P<0.01).Compared with the MS-OA group,the contents of serum TC,TG,LDL-C,TNF-α and leptin decreased in TCM high-dosage group(P<0.05,P<0.01),the content of IL-10 increased(P<0.05),the pathological damage of cartilage tissue improved,the Mankin score decreased(P<0.01),the expressions of ColⅩ,MMP13,TNF-α,IL-1β and p-NF-κBp65 protein in cartilage tissue decreased(P<0.05,P<0.01),and the protein expressions of Acan and PPARγ protein increased(P<0.01,P<0.05).Conclusion Fangji Huangqi Xiaozhong Prescription can improve lipid metabolism disorder,improve intra-articular inflammatory environment,balance cartilage metabolism,and delay cartilage degeneration in MS-OA rats.Its mechanism may be related to the regulation of PPARγ/NF-κB signaling pathway.关键词
防己黄芪消肿方/骨关节炎/代谢综合征/PPARγ/NF-κB信号通路/大鼠Key words
Fangji Huangqi Xiaozhong Prescription/osteoarthritis/metabolic syndrome/PPARγ/NF-κB signaling pathway/rats分类
医药卫生引用本文复制引用
危一飞,李紫阁,白天宇,邱佳明,王宏杰,肖潇,温冠楠,梁佩文,程桯..基于PPARγ/NF-κB信号通路探讨防己黄芪消肿方干预代谢综合征表型骨关节炎作用机制[J].中国中医药信息杂志,2024,31(8):76-83,8.基金项目
中国中医科学院望京医院基础研究苗圃培育计划(WJYY-YJKT-2022-08) （WJYY-YJKT-2022-08）
国家自然科学基金青年科学基金(82305277) （82305277）
首都卫生发展科研专项(首发-2022-2-4162) （首发-2022-2-4162）
中国中医科学院骨伤科学重大攻关项目(CI2021A02001) （CI2021A02001）
中国中医科学院高水平中医医院建设项目(WJZJ-202322) （WJZJ-202322）
