中国中医药信息杂志2024，Vol.31Issue(8)：97-102,6.DOI:10.19879/j.cnki.1005-5304.202401545
涤痰益智汤对血管性痴呆大鼠海马神经元形态及FAM134B蛋白表达的影响
Effects of Ditan Yizhi Decoction on Neuronal Morphology and FAM134B Protein Expression in Hippocampus of Vascular Dementia Rats
摘要
Abstract
Objective To observe the effects of Ditan Yizhi Decoction on learning and memory ability,structure of hippocampal tissue,neuronal morphology of hippocampus,and the expression of endoplasmic reticulum autophagy-related protein FAM134B in hippocampal tissue;To explore the mechanism of its therapeutic effect on vascular dementia.Methods Totally 32 SD rats were randomly divided into sham-operation group,model group,donepezil group and Ditan Yizhi Decoction group,with 8 rats in each group.The model group,donepezil group and Ditan Yizhi Decoction group were prepared with a modified permanent ligation method of bilateral common carotid arteries to create a rat model of vascular dementia,the common carotid artery was separated in the sham-operation group,but not ligated.After modeling,the donepezil group was given donepezil hydrochloride,Ditan Yizhi Decoction group was given Ditan Yizhi Decoction,and the sham-operation group and model group were given equal volume of distilled water for gavage for 4 consecutive weeks.Morris water maze experiment was used to evaluate the learning and memory ability,HE staining and Nissl staining were used to observe the morphological changes of hippocampus,ultrastructure of hippocampal neurons was observed using transmission electron microscopy,Western blot was used to detect the protein expression of FAM134B and p-FAM134B in hippocampal tissue.Results Compared with the sham-operation group,the escape latency period was prolonged of the rats in model group,and the number of crossing the original platform and the duration of stay in the target quadrant was reduced(P<0.01),the gap between neurons in CA1 region of the hippocampus increased,the cell morphology was irregular,the boundaries were blurred,the neurons shrinked,the Nissl bodies dissolved and broke,the number decreased,the endoplasmic reticulum arrangement was scattered,mitochondria swelled and deformed,and the expressions of FAM134B and p-FAM134B protein in hippocampal tissue increased(P<0.01).Compared with the model group,the escape latency period of rats in donepezil group and Ditan Yizhi Decoction group were significantly shortened,and the number of crossing the original platform and the duration of stay in the target quadrant were increased(P<0.01),the morphology and quantity of neurons in CA1 region of the hippocampus were more regular,with a decrease in neuronal pyknosis,an increase in the number of Nissl bodies,and a reduction in dissolution and fragmentation,the swelling and deformation of the endoplasmic reticulum were restored,and the expression of FAM134B and p-FAM134B protein in hippocampal tissue increased(P<0.01).Moreover,the effects of Ditan Yizhi Decoction group were better than those of the donepezil group(P<0.01).Conclusion Ditan Yizhi Decoction can improve the learning and memory ability and the morphology of neurons in vascular dementia rats.The mechanism may related to increasing the expression and phosphorylation of FAM134B protein,thereby promoting endoplasmic reticulum autophagy.关键词
血管性痴呆/涤痰益智汤/FAM134B/内质网自噬/神经元Key words
vascular dementia/Ditan Yizhi Decoction/FAM134B/endoplasmic reticulum autophagy/neuron分类
医药卫生引用本文复制引用
顾梦宇,贺延妮,张思怡,孙烈乾,杨佳,杨超,杨杰,郭高帅,王堂龙,杨志伟,王凯漪..涤痰益智汤对血管性痴呆大鼠海马神经元形态及FAM134B蛋白表达的影响[J].中国中医药信息杂志,2024,31(8):97-102,6.基金项目
湖北省中医药管理局中医药青年人才项目(ZY2023Q010) （ZY2023Q010）
湖北省自然科学基金面上项目(2023AFB915) （2023AFB915）
湖北省卫生健康委员会卫生健康科研立项面上项目(WJ2023M097) （WJ2023M097）
鄂州市科技局基础人才与创新专项(EZ01-007-20230100) （EZ01-007-20230100）
湖北省中西医结合医院"杏林人才工程"青年项目(H2023Q009) （H2023Q009）
