中国中医药信息杂志2024，Vol.31Issue(8)：126-132,7.DOI:10.19879/j.cnki.1005-5304.202310552
UPLC指纹图谱结合化学计量学分析和多指标成分含量测定的羌活药材质量评价研究
Study on Quality Evaluation of Notopterygii Rhizoma et Radix through UPLC Fingerprint Combined with Chemometrics Analysis and Multi Index Component Content Determination
摘要
Abstract
Objective To establish the ultra-high performance liquid chromatography(UPLC)chromatographic fingerprint of Notopterygii Rhizoma et Radix;To determine the contents of ferulic acid,nodakenin,ammijin,notopterol,isoimperatorin and volatile oil of Notopterygii Rhizoma et Radix from different producing areas;To provide reference for quality evaluation of Notopterygii Rhizoma et Radix.Methods Waters BEH C18 chromatographic column(2.1 mm×150 mm,1.7 µm)was used,with mobile phase acetonitrile-0.02%formic acid aqueous solution gradient elution,flow rate 0.25 mL/min,column temperature 25℃,detection wavelength 330 nm,injection volume 2 µL.UPLC fingerprints of 25 batches of Notopterygii Rhizoma et Radix were established,and the similarity analysis and chemometrics analysis were carried out.The contents of ferulic acid,nodakenin,ammijin,notopterol and isoimperatorin were determined simultaneously,and the contents of volatile oil was determined by steam distillation method.Results Totally 23 common fingerprint peaks were calibrated,11 known components were identified.According to the results of the cluster analysis and principal component analysis,25 batches of Notopterygii Rhizoma et Radix samples were divided into 3 categories,and the 6 potential differential components were screened out by orthogonal partial least squares-discriminant analysis(OPLS-DA).The results showed that the contents of notopterol and volatile oil from Sichuan Province were higher than those from Gansu Province and Qinghai Province.Conclusion The method established in the study is accurate and reliable,which can provide scientific basis and reference for the quality evaluation and control of Notopterygii Rhizoma et Radix.关键词
羌活/指纹图谱/多指标成分/含量测定/超高效液相色谱法Key words
Notopterygii Rhizoma et Radix/fingerprint/multi index component/content determination/UPLC分类
医药卫生引用本文复制引用
何广铭,张坚祥,陈向东,孙冬梅,卢晓莹,夏天睿,谢翡翡,罗睿,吕渭升,梁月仪,李振雨,李秀枝..UPLC指纹图谱结合化学计量学分析和多指标成分含量测定的羌活药材质量评价研究[J].中国中医药信息杂志,2024,31(8):126-132,7.基金项目
国家工业和信息化部产业技术基础公共服务平台项目-中药全产业链质量技术服务平台(2022-230-221) （2022-230-221）
佛山市南海区重点领域科技攻关专项(2022年) （2022年）
