中成药2024，Vol.46Issue(7)：2129-2133,5.DOI:10.3969/j.issn.1001-1528.2024.07.001
北柴胡质量控制研究
Quality contol of Bupleurum chinense
摘要
Abstract
AIM To control the quality of Bupleurum chinense DC.METHODS The analysis was performed on a 35℃ thermostatic Venusil XBP C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min,and the detection wavelength was set at 210 nm.The HPLC fingerprints were established,after which the contents of saikosaponin A,saikosaponin B2,saikosaponin C,saikosaponin D,saikosaponin E,saikosaponin F and 6″-O-acetylsaikosaponin A were determined,and principal component analysis was made.RESULTS There were thirteen common peaks in the fingerprints for twelve batches of medicinal materials with the similarities of 0.970-0.995.Seven constituents showed good linear relationships within their own ranges(R2≥0.999 8),whose average recoveries were 90.75%-100.91% with the RSDs of 1.6%-4.0% .Various constituents demonstrated similar contents in medicinal materials originated in Inner Mongolia and Shanxi.CONCLUSION This precise,accurate and stable method can be used for the quality evaluation of B.chinense.关键词
北柴胡/质量控制/HPLC指纹图谱/含量测定/主成分分析Key words
Bupleurum chinense DC./quality control/HPLC fingerprints/content determination/principal component analysis分类
医药卫生引用本文复制引用
于静波,韩越,艾启迪,孙洋,周梓洋,刘赛虎,张子浩,舒慧,王宇红..北柴胡质量控制研究[J].中成药,2024,46(7):2129-2133,5.基金项目
国家自然科学基金项目(82204613) （82204613）
中药粉体与创新药物省部共建国家重点实验室培育基地开放基金项目(科函[2020]8号) （科函[2020]8号）
湖南省自然科学基金项目(2020JJ5415,2021JJ40410) （2020JJ5415,2021JJ40410）
湖南中医药大学一流学科开放基金项目(2020ZYX06,2021ZYX25) （2020ZYX06,2021ZYX25）
