中成药2024，Vol.46Issue(7)：2140-2146,7.DOI:10.3969/j.issn.1001-1528.2024.07.003
UPLC-MS/MS法同时测定阿胶中18种核苷、游离氨基酸的含量
Simultaneous content determination of eighteen nucleosides and free amino acids in Colla corii asini by UPLC-MS/MS
摘要
Abstract
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of Asp,Guad,Adeno,Arg,Ade,Cyti,Phe,Leu,Ile,Glu,Ser,Gln,Gly,Ala,Hyp,Thr,Pro and Lys in Asini Corii Colla.METHODS The analysis was performed on a 45℃ thermostatic Waters BEH C18column(2.1 mm×50 mm,1.7 μm),with the mobile phase comprising of acetonitrile(containing 0.1% formic acid)-water flowing at 0.35 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive ion scanning with multiple reaction monitoring mode.Subsequently,chemical pattern recognition was performed by hierarchical clustering analysis,principal component analysis and orthogonal partial least squares-discriminant analysis.RESULTS Eighteen nucleosides and free amino acids showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 98.0%-104.9% with the RSDs of 1.6%-4.9% .Seventeen batches of samples were clustered into two categories,two principal components demonstrated the accumulative variance contribution rate of 60.75%,Leu,Phe,Ade and Guad were potential index constituents.CONCLUSION This stable and reliable method can be used for the quality control of Asini Corii Colla.关键词
阿胶/核苷/游离氨基酸/含量测定/UPLC-MS/MS/化学模式识别Key words
Asini Corii Colla/nucleosides/free amino acids/content determination/UPLC-MS/MS/chemical pattern recognition分类
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杨帅,郑林,迟明艳,巩仔鹏,李月婷,魏茂陈,黄勇..UPLC-MS/MS法同时测定阿胶中18种核苷、游离氨基酸的含量[J].中成药,2024,46(7):2140-2146,7.基金项目
贵州省科技计划项目(黔科合支撑[2021]419号,黔科合平台人才-GCC[2022]031-1号,黔科合平台人才-CXTD[2023]019号) （黔科合支撑[2021]419号,黔科合平台人才-GCC[2022]031-1号,黔科合平台人才-CXTD[2023]019号）
