中成药2024，Vol.46Issue(7)：2157-2163,7.DOI:10.3969/j.issn.1001-1528.2024.07.006
不同工艺对养肝降草合剂整体质量的影响
Effects of different processes on the overall quality of Yanggan Jiangcao Mixture
摘要
Abstract
AIM To investigate the effects of extraction,concentration,centrifugation,sterilization processes on overall quality of Yanggan Jiangcao Mixture.METHODS The analysis was performed on a 20℃ thermostatic Phenomenex Luna C18column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1 mL/min in a gradient elution manner.The HPLC fingerprints were established,after which the contents of salidroside,geniposide,forsythoside A,specnuezhenide,glycyrrhizic acid and atractylenolideⅢ,along with the calculation of their transfer rates.RESULTS There were twenty common peaks in the fingerprints for fifteen batches of samples under different processes with the similarities of more than 0.950.Six constituents showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 96.96%-106.69% with the RSDs of 0.97%-2.87%,and the transfer rates and total transfer rates were more than 80% and more than 90%,respectively.CONCLUSION Yanggan Jiangcao Mixture demonstrates good stability under different processes.关键词
养肝降草合剂/提取工艺/浓缩工艺/离心工艺/灭菌工艺/HPLC指纹图谱/含量测定Key words
Yanggan Jiangcao Mixture/extraction process/concentration process/centrifugation process/sterilization process/HPLC fingerprints/content determination分类
医药卫生引用本文复制引用
刘玉鑫,朱亚苹,田立元,吴磊..不同工艺对养肝降草合剂整体质量的影响[J].中成药,2024,46(7):2157-2163,7.基金项目
国家中医药管理局2023年中药创新能力提升项目(k2023BZ04) （k2023BZ04）
江苏省中医院院级课题(Y22051) （Y22051）
江苏省中医院科主任提升项目(Y2022ZR19) （Y2022ZR19）
