中成药2024，Vol.46Issue(7)：2163-2172,10.DOI:10.3969/j.issn.1001-1528.2024.07.007
UPLC-QTOF/MS法分析乳宁颗粒化学成分及入血成分
Analyses of chemical constituents and constituents absorbed into blood from Runing Granules by UPLC-QTOF/MS
摘要
Abstract
AIM To establish a UPLC-QTOF/MS method for the analyses of chemical constituents and constituents absorbed into blood from Runing Granules.METHODS Two rats were given intragastric administration of the aqueous solution of granules,after which blood collection was made at 0,0.5,1,2,4 h.The analysis was performed on a 40℃ thermostatic Acquity UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm),with the mobile phases comprising of 0.1% formic aci D-acetonitrile(positive ion)or water-acetonitrile(negative ion)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning.RESULTS Total 166 chemical constituents were identified,containing 44 flavonoids,17 terpenes,23 alkaloids,11 saponins,29 phenolic acids,13 quinones,10 organic acids,and 19 others,along with 11 constituents absorbed into blood.CONCLUSION This accurate and stable method can provide a data support for the clinical application of Runing Granules,and lay foundations for the further researches on their quality control and material basis.关键词
乳宁颗粒/化学成分/入血成分/UPLC-QTOF/MSKey words
Runing Granules/chemical constituents/constituents absorbed into blood/UPLC-QTOF/MS分类
医药卫生引用本文复制引用
彭秘,张凌云,刘匡一..UPLC-QTOF/MS法分析乳宁颗粒化学成分及入血成分[J].中成药,2024,46(7):2163-2172,10.基金项目
江西省中医药管理局科技计划项目(2023B1357) （2023B1357）
