中成药2024，Vol.46Issue(7)：2182-2188,7.DOI:10.3969/j.issn.1001-1528.2024.07.010
基于PTEN/PI3K/Akt信号通路探讨糖通饮对糖尿病肾病大鼠足细胞损伤的保护作用
Protective effects of Tangtongyin on podocyte injury in rats with diabetic nephropathy via PTEN/PI3K/Akt signaling pathway
摘要
Abstract
AIM To investigate the protective effects of Tangtongyin on podocyte injury in rats with diabetic nephropathy(DN).METHODS The DN rat models established by feeding of high-fat and high-sugar diet combined with multiple injections of streptozotocin(STZ)were randomly divided into the model group,the irbesartan group(13.5 mg/kg)and the low-dose,medium-dose and high-dose Tangtongyin groups(930,1 860,3 720 mg/kg),in contrast to those normal rats of the blank group.In the 8 weeks consecutive administration,each group was intragastrically dosed with the corresponding drug once daily.The rats had their fasting blood glucose(FBG),24-hour urine protein(24-h UP),serum creatinine(Scr)and blood urea nitrogen(BUN)levels detected;their renal pathological changes observed by HE staining;their ultrastructural changes of renal podocytes observed by transmission electron microscopy;their mRNA expressions of renal PTEN,PI3K,Akt,nephrin,podocin and CD2AP detected by RT-qPCR;and their protein expressions of renal PTEN,PI3K,Akt,p-Akt,nephrin,podocin and CD2AP detected by Western blot.RESULTS Compared with the model group,the Tangtongyin groups displayed decreased levels of FBG,24-h UP,Scr and BUN(P＜0.05,P＜0.01);improved renal pathological morphology and podocyte ultrastructure;increased mRNA and protein expressions of PTEN,nephrin,podocin and CD2AP(P＜0.05,P＜0.01);and decreased expression of p-Akt protein(P＜0.05,P＜0.01).Additionally,the medium-dose and high-dose Tangtongyin groups shared indecreased mRNA and protein expressions of PI3K and Akt(P＜0.05,P＜0.01).CONCLUSION Tangtongyin can protect the renal podocytes in DN rats,and its mechanism may associate with its efficacy via PTEN/PI3K/Akt signaling pathway.关键词
糖通饮/糖尿病肾病/足细胞/PTEN/PI3K/Akt信号通路Key words
Tangtongyin/diabetic nephropathy/podocyte/PTEN/PI3K/Akt signaling pathway分类
医药卫生引用本文复制引用
杨红,潘艳伶,陈洪民,伏红颖,唐露,凌湘力..基于PTEN/PI3K/Akt信号通路探讨糖通饮对糖尿病肾病大鼠足细胞损伤的保护作用[J].中成药,2024,46(7):2182-2188,7.基金项目
国家自然科学基金项目(82060837) （82060837）
贵州省科技基金委员会合作计划项目(黔科合基础-ZK[2023]一般369号) （黔科合基础-ZK[2023]一般369号）
2024年度贵州省中医药管理局中医药、民族医药科学技术研究专项课题(QZYY-2024-025) （QZYY-2024-025）
凌湘力全国名中医传承工作室建设项目(国中医药办人教函[2022]245号) （国中医药办人教函[2022]245号）
