中成药2024，Vol.46Issue(7)：2211-2219,9.DOI:10.3969/j.issn.1001-1528.2024.07.014
制何首乌对脑卒中后抑郁大鼠神经功能的改善作用
Effects of Polygoni multiflori Radix Preparata on the neurological function improvement in a rat model of post-stroke depression
摘要
Abstract
AIM To explore the effects and mechanism of Polygoni multiflori Radix Preparata(PMRP)ona rat model of post-stroke depression(PSD).METHODS The models of middle cerebral artery occlusion(MCAO)established by thread embolism method were then randomly divided into the model group,the positive drug(fluoxetine)group,and the low-dose and high-dose PMRP groups,with 10 rats in each group,in contrast to the 10 rats of the sham operation group.After 7 days of MCAO modeling,the rats underwent their 21-day PSD modeling except those of the sham operation group,during which the rats had their the neurological functions and behaviors assessed on the 1st,7th,14th and 21st day;and their cerebral infarction area and brain water content detected on the 21st day.HE staining,Nissl staining and immunohistochemical staining were used to observe the pathological morphology of cerebral ischemic penumbra.ELISA and Western blot were applied in the detections of the cerebral protein expressions of aquaporins(AQP3,AQP4,AQP5)and brain-derived neurotrophic factor(BDNF).RESULTS Compared with the model group,the high-dose PMRP group displayed improved neurological functions and behavioral scores(P＜0.05,P＜0.01),and reduced cerebral infarction area and brain water content(P＜0.05).Compared with the model group,each treatment group demonstrated clearer brain structure in ischemic penumbra,smaller intercellular space,increased neuron counts,decreased cerebral protein expressions of AQP3,AQP4 and AQP5(P＜0.05,P＜0.01),and increased BDNF protein expression(P＜0.05).CONCLUSION PMRP can reduce the depression of PSD rats by improving the microenvironment of neurons to promote their growth and survival,and eliminating the brain edema to enhance neurological functions via reduced protein expressions of AQP3,AQP4 and AQP5,and increased BDNF protein expression.关键词
制何首乌/脑卒中后抑郁/水通道蛋白/脑源性神经营养因子Key words
Polygoni multiflori Radix Preparata/post-stroke depression/aquaporins/brain-derived neurotrophic factor分类
医药卫生引用本文复制引用
刘洋,田洋,冷沁洁,彭静,吴晓青,谭睿..制何首乌对脑卒中后抑郁大鼠神经功能的改善作用[J].中成药,2024,46(7):2211-2219,9.基金项目
四川省中医药产业发展重大项目(510201202109711) （510201202109711）
四川省中医药管理局团队项目(2022C010) （2022C010）
成都市科技项目"技术创新研发项目"(2021-YF05-00447-SN) （2021-YF05-00447-SN）
