中成药2024，Vol.46Issue(7)：2259-2265,7.DOI:10.3969/j.issn.1001-1528.2024.07.023
盐橘核化学成分及其抗氧化活性
Chemical constituents from salt-processed Citri Reticulatae Semen and their antioxidant activities
摘要
Abstract
AIM To study the chemical constituents from salt-processed Citri Reticulatae Semen and their antioxidant activities.METHODS The 85% ethanol extract from salt-processed Citri Reticulatae Semen was isolated and purified by silica gel,D101 macroporous resins,MCI,ODS,Sephadex LH-20 and semi-prepative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their antioxidant activities in vitro of the ethanol extract of Citri Reticulatae Semen,salt-processed Citri Reticulatae Semen and all the obtained compounds were evaluated by DPPH and ABTS+assay.RESULTS Sixteen compounds were isolated and identified as limonin(1),obacunone(2),nomilin(3),deacetyl nomilin(4),kaempferol(5),nobiletin(6),diosmetin(7),isosakuranetin(8),hesperetin(9),epicatechin(10),trans-p-menthane-1α,2β,8-triol(11),byakangelicin(12),vanillin(13),p-coumaric acid(14),4-[(1-ethoxy-2-hydroxy)ethyl]phenol(15),catechol(16).Compound 10 showed strong DPPH free radical scavenging activity,with an IC50 value of(0.015±0.001)μmol/mL,and strong ABTS+radical scavenging activity,with an IC50 value of(0.010±0.005)μmol/mL.CONCLUSION Compounds 8,11,15-16 are isolated from genus Citrus for the first time,5,12,14 are obtained from Citri Reticulatae Semen for the first time.Compound 10 shows obvious antioxidant activities.After salt roasting,the antioxidant activity of the ethanol extract of Citri Reticulatae Semen is enhanced.关键词
盐橘核/化学成分/分离鉴定/抗氧化活性Key words
salt-processed Citri Reticulatae Semen/chemical constituents/isolation and identification/antioxidant activities分类
医药卫生引用本文复制引用
杨兴,陈章贤,王一谋,邓徐丽,张洪川,尹蕊,董维茂,何红平,董发武..盐橘核化学成分及其抗氧化活性[J].中成药,2024,46(7):2259-2265,7.基金项目
国家自然科学基金项目(31860092,82260758) （31860092,82260758）
云南省高层次人才培养支持计划"青年拔尖人才"专项项目(YNWR-QNBJ-2020-255) （YNWR-QNBJ-2020-255）
云南省基础研究计划面上项目(202101AT070254) （202101AT070254）
云南省南药可持续利用研究重点实验室2021年开放课题重点项目(202105AG070012ZD2201) （202105AG070012ZD2201）
