中成药2024，Vol.46Issue(7)：2272-2277,6.DOI:10.3969/j.issn.1001-1528.2024.07.025
刀豆化学成分及其雌激素样活性研究
Chemical constituents from the seeds of Canavalia gladiata and their estrogen-like activities
摘要
Abstract
AIM To study the chemical constituents from seeds of Canavalia gladiata(Jacq.)DC.,and their estrogen-like activities.METHODS The 75% ethanol extract from the seeds of C.gladiata was isolated and purified by various chromatography,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The estrogen-like activities were evaluated by human breast cancer cells(MCF-7)stably transfected with estrogen receptor reporter gene(ER-Luc).RESULTS Twelve compounds were isolated and identified as biorobin(1),kaempferol 3-O-[O-β-D-apio-D-furanosyl(1→2)-O-[α-L-rhamno-pyranosyl(1 → 6)]-β-D-galactopyranoside(2),kaempferol 3-O-{β-D-glucopyranosyl(1 → 2)-[α-L-rhamonopyranosyl(1→6)]-β-D-galactopyranoside}(3),iso-quercitrin(4),quercetin 3-O-(6″-O-galloyl)-β-D-glucoside(5),isomyricitrin(6),myricetin-3-O-rutinoside(7)(3-ethenylphenyl)-1,2-ethanediol(8),1,3,6-tri-O-galloyl-β-D-glucopyranose(9),breynioside A(10),4-hydroxy-3-methoxyphenol-1-O-β-D-(6′-O-galloyl)glucoside(11),maesopsin-6-O-β-D-glucopyranoside(12).Compounds 1,3-5,8-9 in the concentration of 50 μmol/L,and compounds 2,12 in 100 μmol/L showed estrogen-like activities.CONCLUSION All compounds are isolated from the gene Canavalia for the first time.Compounds 1,3-5,8-9 showed stronger estrogen-like activities than compounds 2 and 12.关键词
刀豆/化学成分/分离鉴定/雌激素样活性Key words
seeds of Canavalia gladiata(Jacq.)DC./chemical constituents/isolation and identification/estrogen-like activities分类
医药卫生引用本文复制引用
吕紫阳,闫若雨,刘拯珲,杨颖博,张紫佳..刀豆化学成分及其雌激素样活性研究[J].中成药,2024,46(7):2272-2277,6.基金项目
上海市自然科学基金项目(22ZR1461300) （22ZR1461300）
