肿瘤防治研究2024，Vol.51Issue(7)：546-553,8.DOI:10.3971/j.issn.1000-8578.2024.23.1258
慢病毒介导CIB1表达下调对乳腺癌细胞生物学行为的影响
Effects of Lentivirus-Mediated CIB1 Silencing on Biological Behavior of Breast Cancer Cells
摘要
Abstract
Objective To investigate the effects of calcium and integrin-binding protein 1(CIB1)on the cell proliferation,invasion,apoptosis,and migration of triple-negative breast cancer cells and its possible mechanism.Methods MDA-MB-231 and MDA-MB-468 cells were divided into CIB1-knockdown(infected with CRISPR/Cas9 lentivirus)and negative-control groups.Cell Counting Kit-8(CCK-8)and 5-ethynyl-2′-deoxyuridine(EdU)assays were performed to detect cell proliferation.Cell apoptosis was determined through flow cytometry.Scratch and Transwell experiments were conducted to measure the migration and invasion abilities of cells.The mRNA and protein expression levels of β-catenin,adenomatous polyposis coli(APC),glycogen synthase kinase 3β(GSK-3β),and c-myc were detected via real-time quantitative polymerase chain reaction and Western blot.Results Compared with the negative-control group,the CIB1-knockdown group showed decreased cell proliferation,invasion,and migration(P<0.05)and increased cell apoptosis(P<0.05).The mRNA and protein expressions of β-catenin,APC,and c-myc decreased(P<0.05),and that of GSK-3β increased(P<0.05).Conclusion CIB1 knockdown can inhibit cell proliferation,invasion,and migration and promote the apoptosis of breast cancer cells.Its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.关键词
CIB1/三阴性乳腺癌/增殖/迁移/侵袭/Wnt/β-catenin信号通路Key words
CIB1/Triple negative breast cancer/Proliferation/Migration/Invasion/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
贾庆华,王晓宇,张富婷,马俊,牛廷献..慢病毒介导CIB1表达下调对乳腺癌细胞生物学行为的影响[J].肿瘤防治研究,2024,51(7):546-553,8.基金项目
Laboratory Cultivation Project of the 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army(No.2021yxky083) 联勤保障部队第九四〇医院实验室培育项目(2021yxky083) （No.2021yxky083）
