中兽医医药杂志2024，Vol.43Issue(4)：19-25,7.DOI:10.13823/j.cnki.jtcvm.2024.053
基于HPLC特征图谱结合化学模式识别法评价彝药鸡根饮片质量
Quality control of Jigen pieces using HPLC characteristic chromatogram combined with chemical pattern recognition method
摘要
Abstract
The objective was to establish a quality control method for Jigen pieces based on characteristic chromatogram and component determination.Agilent ZORBAX Extend-C18(4.6 mm×250.0 mm,5 μm)column was used for gradient elution with methanol-0.1%phosphoric acid solution,wavelength of 330 nm,flow rate of 1.0 mL/min,column temperature of 30℃,and injection volume of 5 μL.The characteristic chromatogram was established by the Similarity Evaluation System of Traditional Chinese Medicine Fingerprint,and the main differential components of Jigen pieces between different batches were analyzed by cluster analysis,principal component analysis and orthogonal partial least squares discrimination,and the content of 3,6'-diglucoylsucrose was determined.Based on the characteristics of 12 batches of Jigen pieces in Yunnan producing area,four common peaks were determined,and one component was identified as 3,6'-diglucoylsucrose,and the similarity of the characteristic maps was greater than 0.860.Cluster analysis divided the samples into two categories:principal component analysis(PCA)was used to screen out two principal components with eigenvalues greater than 1,and orthogonal partial least squares discriminant analysis(VIP>1)was used to screen out two landmark components with mass differences,such as 3,6'-diglucoylsucrose.The average recovery rate was 98.683%and the RSD was less than 3.00%,the results showed that there were differences in the content of 3,6'-diglucoylsucrose in 15 batches of Jigen pieces from five producing areas.Among them,the content of 3,6'-diglucoylsucrose in Simao area was the highest.The characteristic map and content determination method of Jigen pieces established in this study are accurate,stable and reliable,which can provide a reference for controlling and evaluating the quality of Jigen pieces.关键词
鸡根/特征图谱/含量测定/3,6'-二芥子酰基蔗糖/化学模式识别Key words
Jigen/characteristic chromatogram/component determination/3,6'-diglucoylsucrose/chemical recognition pattern分类
农业科技引用本文复制引用
叶丽娟,徐素娥,刁振慧,杨国春,杨亚芳,柯瑾,陈凌云,侯安国,周紫婧..基于HPLC特征图谱结合化学模式识别法评价彝药鸡根饮片质量[J].中兽医医药杂志,2024,43(4):19-25,7.基金项目
云南省重大科技专项(202102AA310027) （202102AA310027）
云南省中医药学分子生物学重点实验室(2019DG016) （2019DG016）
云南省基础研究专项(202001AU070133) （202001AU070133）
云南省中医联合专项(202001AZ070001-091) （202001AZ070001-091）
