中兽医医药杂志2024，Vol.43Issue(4)：48-53,封2,7.DOI:10.13823/j.cnki.jtcvm.2024.045
基于网络药理学与分子对接探讨金银花对鸡禽流感的潜在作用机制
Exploration on the potential action mechanism of Flos Lonicerae in avian influenza in chickens based on network pharmacology and molecular docking
摘要
Abstract
To explore the potential action mechanism of Flos Lonicerae in avian influenza in chickens through network pharmacology and molecular docking.The active ingredients and corresponding targets of Flos Lonicerae were obtained based on the Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform(TCMSP),and then the targets of the diseases were searched and integrated by GeneCard,OMIM and TTD databases,and the intersecting targets of Flos Lonicerae and chicken avian influenza were analyzed using the STRING database,and the PPI network diagram was established.Core targets in the PPI network were screened in Cytoscape 10.1.1 software,and these targets were analyzed for GO function annotation and KEGG pathway enrichment in the DAVID database.The results showed that the main active components of Flos Lonicerae were screened as beta-carotene,luteolin,quercetin,and kaempferol,and there were 33 common targets with the disease,and the PPI network showed that the core targets were B lymphoblastoma-2 gene(BCL-2),albumin(ALB),MYC transcription factor(MYC),cystatinase 3(CASP-3),interleukin-6(IL-6)and transforming growth factor β1(TGF-β1),among others.GO functional annotation analysis resulted in 28 entries for biological processes,3 entries for cellular components,and 6 entries for molecular functions(P<0.05).KEGG pathway enrichment analysis yielded a total of 15 signaling pathways associated with avian influenza in chickens(P<0.05).The results of molecular docking showed that luteolin and BCL-2,ALB as well as beta-carotene and IL-6 exhibited good binding.The results indicated that the main active components of Flos Lonicerae,beta-carotene,luteolin,quercetin,and kaempferol,might play a role through the core targets of BCL-2,ALB,MYC,CASP-3,IL-6,and TGF-β1,as well as the related TLRs signaling pathway,MAPK signaling pathway,CLRs signaling pathway,and FoxO signaling pathway,playing an important role in the prevention of avian influenza in chickens.关键词
金银花/禽流感/网络药理学/分子对接/木犀草素/作用机制Key words
Flos Lonicerae/avian influenza/network pharmacology/molecular docking/luteolin/action mechanism分类
农业科技引用本文复制引用
肖明月,侯永威,高雲,韦春波..基于网络药理学与分子对接探讨金银花对鸡禽流感的潜在作用机制[J].中兽医医药杂志,2024,43(4):48-53,封2,7.基金项目
黑龙江八一农垦大学大学生创新创业训练项目(XC2023008) （XC2023008）
