中兽医医药杂志2024，Vol.43Issue(4)：54-57,4.DOI:10.13823/j.cnki.jtcvm.2024.036
基于HPLC-MS/MS对海博麦布中对甲苯胺、对氟苯胺、邻氟苯胺的残留检测研究
Residual detection of 4-aminotoluene,4-fluoroaniline and 2-fluoroaniline in hybutimibe by HPLC-MS/MS
摘要
Abstract
A high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC-MS/MS)method was established to determinate the residues of 4-aminotoluene,4-fluoroaniline and 2-fluoroaniline in hybutimibe.The compounds were separated by high performance liquid chromatography with Waters ACQUITY UPLC® BEH C18(2.1 mm×100.0 mm,1.7 μm)column.0.1%formic acid solution was used as the mobile phase A and methanol as the mobile phase B in gradient elution.The detector was triple quadrupole mass spectrometry.The MS data for the compounds were collected by positive ion electrospray ionization(ESI)using multi reaction monitoring mode(MRM).The linear ranges of 4-aminotoluene,4-fluoroaniline and 2-fluoroaniline were 1.000 0-20.000 0 ng/L,correlation coefficients(r)were 0.997 5-0.999 9.The detection limit concentrations were 0.33 mg/kg,and the quantification limit concentrations were 1.00 mg/kg.The method recovery rates were 85%-95%.The method is simple with good linear relationship,low detection limit and high recovery rate,which provide a technical support for the quality control of 4-aminotoluene,4-fluoroaniline and 2-fluoroaniline in hybutimibe.关键词
高效液相色谱/三重四极杆质谱/对甲苯胺/对氟苯胺/邻氟苯胺/残留检测Key words
high performance liquid chromatography/triple quadrupole mass spectrometry/4-aminotoluene/4-fluoroaniline/2-fluoroaniline/residual research分类
农业科技引用本文复制引用
和燕玲,杨荷友,吴春艳,潘妮娜,李于西,邱卫晓,赵燕,苟金霞,邵伍军..基于HPLC-MS/MS对海博麦布中对甲苯胺、对氟苯胺、邻氟苯胺的残留检测研究[J].中兽医医药杂志,2024,43(4):54-57,4.基金项目
浙江省2023年度高校国内访问工程师校企合作项目(FG2023323) （FG2023323）
