中兽医医药杂志2024，Vol.43Issue(4)：73-77,5.DOI:10.13823/j.cnki.jtcvm.2024.044
板青颗粒特征图谱及其质量相关性研究
Characteristic chromatogram and quality correlation of Banqing granules
摘要
Abstract
The objective was to develop a method for content determination of indirubin in Banqing granules and conduct fingerprint chromatogram.High performance liquid chromatography was used with Eclipse Plus C18 column(4.6 mm×250.0 mm,5 μm).Detection wavelength was 289 nm using methanol-water(77∶23)as the mobile phase in equimolar elution.The flow rate was 0.8 mL/min with column temperature of 30℃and injection volume of 10 μL.The results showed that in the qualitative analysis of indirubin,the linear relationship was good(R2=1.000 0),and the average recovery rate was 98.07%.A total of 7 peaks were detected in 12 batches of samples with a similarity of 0.791-1.000.The experimental operation is simple,fast,and the results are accurate.It can be used for the determination of indirubin content in Banqing granules,and for the study of characteristic chromatogram and quality evaluation of Banqing granules.关键词
板青颗粒/靛玉红/质量评价/指纹图谱Key words
Banqing granules/indirubin/quality evaluation/fingerprint chromatogram分类
农业科技引用本文复制引用
周景瑞,艾蓉,姜玲玲,冉江,罗文菊,张琴,章厉劼,余波..板青颗粒特征图谱及其质量相关性研究[J].中兽医医药杂志,2024,43(4):73-77,5.基金项目
贵州省科技厅项目(黔科合成果[2021]一般039、黔科合成果[2022]一般008) （黔科合成果[2021]一般039、黔科合成果[2022]一般008）
贵州省农业农村厅项目(GZCYTX-0302) （GZCYTX-0302）
