基于大刍草渗入系的玉米抗旱优异等位基因挖掘OA北大核心CSTPCD

Drought is one of the major abiotic stresses affecting maize production.In order to explore new genes for drought tol-erance in maize,the BC2F6 population constructed on the basis of teosinte and PH4CV was screened for the drought-tolerant introgression lines TP180 through the preliminary identification of drought tolerance at seedling stage.After drought stress,the TP180 was less wilting than its recurrent parent PH4CV,and the survival rate of TP180 was significantly higher than PH4CV after rehydration.Genome-wide genotypic identification revealed that introgression line TP180 contained 0.6%of the teosinte genome.By transcriptomic analysis of TP180 and PH4CV under different water conditions,a total of 2307 differentially expressed genes were identified between TP180 and PH4CV,and 122 of the differentially expressed genes were identified under both two drought stresses conditions.These genes were related to the growth hormone pathway,jasmonic acid pathway,etc.,and contained multiple transcription factors.Integrating the differentially expressed genes and the analysis of introgression region containing the teosinte genome,two drought-resistant candidate genes(Zm00001d033050 and Zm00001d002025)were identified and further validated by RT-PCR.This study provides an important germplasm and information for mining drought-resistant gene of the teosinte.