玉米转录因子ZmEREB180调控根系生长发育及耐逆的功能研究OA北大核心CSTPCD

The AP2/ERF(APETALA2/ethylene-responsive factor)family is one of the largest families of transcription factors in plants,and plays an important role in regulating a variety of biological processes such as plant growth and development,respond-ing to adversity stress,and regulating hormone signaling and substance metabolism.The biological functions of AP2/ERF family genes in many plant species have been validated,but fewer studies have been reported in maize(Zea mays L.).In the previous work,there was significant difference in the relative expression level of the ZmEREB180 transcription factor in root system be-tween the identified critical developmental stages of maize at the six-leaf(V6),the twelve-leaf(V12),and tasseling(VT)stages,the tissue expression analysis revealed that this gene was mainly expressed in maize root system and was significantly higher in young roots than in mature roots,and it was hypothesized that this gene might be involved in the regulation of maize root growth and development.In this study,we cloned the ZmEREB180(Gene ID:100192457)transcription factor gene,and preliminarily analyzed the relative expression pattern and biological functions of ZmEREB180 by bioinformatics,RT-qPCR,subcellular local-ization,and stress-resistant phenotype identification of transgenic Arabidopsis(Arabidopsis thaliana L.)lines.This gene con-tained two exons and the full-length cDNA was 1023 bp,encoding 340 amino acids.The gene had a conserved domain unique to the AP2/ERF family,which expressed most highly in root system of maize;and the gene had different degrees of induced expres-sion under high salt,drought,high nitrogen,and low nitrogen treatment conditions,with a more rapid and higher expression in response to low nitrogen than high nitrogen;root length of ZmEREB180 transgenic Arabidopsis lines were all significantly longer than the wild type(WT)on 1/2 MS medium containing 0.10 mol L-1,0.15 mol L-1 NaCl,and 0.15 mol L-1,0.20 mol L-1,0.30 mol L-1 mannitol(MNT).Under high salt and drought stress conditions in soil environments,transgenic Arabidopsis lines had health-ier growth status,higher green leaf percentage,lower malondialdehyde(MDA)content,and higher peroxidase(POD)activity than WT.The transcription factor ZmEREB180 may play a positive and promotional role in regulating the growth and development of maize root system and enhance the tolerance of maize plants under high salt,drought,osmosis,low nitrogen,and other adversity stresses.This study lays a good foundation for further identification of the biological function and molecular mechanism of tran-scription factor ZmEREB180 in maize.