OsRPTA18参与调控水稻叶片倾角的功能OA北大核心CSTPCD

The regulatory particle triple-A ATPase(RPTA)gene family is closely related to plant growth and development,hor-mone regulation and stress response.In this study,a total of 33 members of the gene family were identified in rice and their gene locations,gene structures,motifs composition,and cis-elements in the promoter regions were revealed.Then,the relative expres-sion patterns of the gene members in OsRPTA family were investigated with the data downloaded from rice CREP database.Most of the OsRPTA genes expressed highly in panicle,endosperm and callus tissues.β-D-glucuronidase(GUS)staining further indi-cated that one of the members,OsRPTA18,was expressed in collar,root,leaf,leaf sheath,stem node,vascular bundles of glume and lemma.Subcellular localization indicated that OsRPTA18 protein was in nucleus.Two knock-out mutants(osrpta18-1 and osrpta18-2)were generated by CRISPR/Cas9 editing technology and both exhibited reduced plant height,leaf inclination,ac-companied by the decreased grain width and 1000-grain weight compared to the wild type Zhonghua 11.Transverse section stain-ing of the lamina joints of the plants indicated the changes in the cell wall thickness and vascular bundle size,causing the obvious proliferation of thick-walled cells at adaxial side,thus decreasing asymmetric development of the lamina joints and the leaf angel in the osrpta18 mutants.These results of this study are valuable for further functional analysis of RPTA genes and utilization of the OsRPTA18 gene for the genetic improvement of rice varieties with ideal plant architecture.