作物学报2024，Vol.50Issue(8)：1989-2000,12.DOI:10.3724/SP.J.1006.2024.34059
利用130K液相芯片分析中国蚕豆种质资源遗传多样性
Genetic diversity analysis of Chinese faba bean(Vicia faba L.)germplasm resources using 130K liquid phase chips
摘要
Abstract
A comprehensive understanding of the genetic diversity and population structure of faba bean(Vicia faba)germplasm resources is of great significance for the mining and innovative utilization of excellent resources.In this study,to analyze the ge-netic diversity,822 Chinese faba bean germplasm resources were genotyped using 130K liquid phase chips,resulting in a total of 30,946 high-quality SNP loci by high-standard filtering.The polymorphism information content(PIC)and Nei's gene diversity were 0.0905-0.3750 and 0.0950-0.5000,with the averages of 0.2600-0.3222,respectively.After evaluating the genetic diversity of 822 accessions using Nei's gene diversity and PIC,based on their geographical origin,the faba bean accessions from Jiangsu and Sichuan had rich genetic diversity,while those from Xinjiang were low.Population structure analysis showed that 822 faba bean accessions were divided into two subpopulations,and when Q was≥0.6,the genetic background of 717 accessions was rela-tively simple.However,the same subpopulation contained materials from different geographical origins,indicating that gene flow or introgression occurred among the accessions.Results from NJ clustering based on Nei's(1972)genetic distance and principal component analysis(PCA)indicated that the 822 accessions were also divided into two subpopulations,which was basically con-sistent with the structure analysis results.In general,the genetic relationships among the faba bean groups with similar ecological environments and geographic origins in neighboring regions or countries were closer and frequently grouped into the same group,while genetic variation among individuals was the main source of the total genetic variation.关键词
液相芯片/蚕豆/遗传多样性/群体结构/遗传变异Key words
liquid phase chips/faba bean/genetic diversity/population structure/genetic variation引用本文复制引用
张红岩,敏玉霞,滕长才,彭小星,陈志凯,周仙莉,娄树宝,刘玉皎..利用130K液相芯片分析中国蚕豆种质资源遗传多样性[J].作物学报,2024,50(8):1989-2000,12.基金项目
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(食用豆,CARS-08),甘肃省自然科学基金项目(22JR5RP1026)和青藏高原种质资源研究与利用实验室开放课题(2022-ZJ-Y01)资助.This study was supported by the China Agriculture Research System of MOF and MARA(Food Legumes,CARS-08),the Natural Science Foundation of Gansu Province(22JR5RP1026),and the Open Project of Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources(2022-ZJ-Y01). （食用豆,CARS-08）
