玉米N-乙酰转移酶ZmNAT1基因响应非生物胁迫的功能分析OA北大核心CSTPCD

The GNAT(Gcn5-related N-acetyltransferase)family proteins play a crucial role in regulating plant growth and deve-lopment,and responding to stress.At present,the biological functions of GNAT family genes have been reported in many species,but there are few studies on its function validation in maize(Zea mays L.).Exploring the functions of maize GNAT family genes not only enriches the genetic resources for maize breeding in China,but also provides an important basis for the creation of new germplasm resources of maize.In this study,the ZmNAT1 gene(Gene ID:541936,GRMZM2G123159)was cloned.Bioinformat-ics analysis showed that the CDS of this gene was 519 bp,encoding 172 amino acids.ZmNAT1 contained a conserved domain unique to GNAT family.The relative expression level of ZmNAT1 gene in different tissues of maize at different stages under dif-ferent stress conditions showed that the expression level of ZmNAT1 was the highest in mature roots,and the relative expression of ZmNAT1 gene could be induced in different degrees under different abiotic adversity stress conditions.Three independent transgenic Arabidopsis(Arabidopsis thaliana L.)pure lines with higher expression were obtained by heterologous expression,and phenotypic characterisation experiments under different adversity stress treatments were carried out on them,the results showed that transgenic Arabidopsis had a better phenotype relative to wild-type Arabidopsis,and the roots of transgenic plants under salt stress,osmotic stress,and drought were significantly longer than those of the wild-type,and the plants showed higher green leaf rate and chlorophyll content,and lower malondialdehyde content than the wild-type plants,which were significant difference.We speculated that ZmNAT1 gene may be involved in response to abiotic stress such as drought and salt in maize.This study provides an important reference for further analysis of the biological functions of ZmNAT1 in maize.