作物学报2024，Vol.50Issue(8)：2143-2156,14.DOI:10.3724/SP.J.1006.2024.34168
卷叶木薯及其突变体叶片的比较转录组分析
Comparative transcriptome profiling of leaf in curled-leaf cassava and its mutant
摘要
Abstract
The objective of this study is to elucidate the molecular regulatory mechanisms and metabolic pathways underlying the abnormal development in the leaf of curled-leaf cassava.Normal curled cassava leaf(JY),mutant expanded leaf(ZY),and mutant curled leaf(BJ)were used as the experimental materials,and bioinformatics analysis was conducted based on transcriptome se-quencing(RNA-seq)data.DESeq differential analysis showed that there were 327(255 up-regulated,72 down-regulated)DEGs for ZY vs BJ,1085(337 up-regulated,748 down-regulated)DEGs for JY vs ZY,and 689(381 up-regulated,308 down-regulated)DEGs for JY vs BJ,and 19 DEGs were co-expressed by three comparative groups.GO functional analysis showed that DEGs had significant differences in the pathways of stimulus response,components of membranes,and transmembrane transporter protein activity.KEGG enrichment analysis indicated that the DEGs were more active in pathways such as phenylpropanoid biosynthesis,phytohormone signaling,and protein processing in the endoplasmic reticulum.Further analysis of the DEGs in the mutated leaf expansion,curled leaves of the same plant,and normal curled leaves of other plants,demonstrated that the DEGs were concen-trated in the phenylpropanoid biosynthesis pathways,starch and sucrose metabolism,and plant hormone signal transduction,which may be the key factors causing leaf expansion mutants.There were 9 main KEGG metabolic pathways and 9 important differentially expressed genes involved in leaf expansion and leaf curling.Microscopic analysis of different types of leaf revealed an increase in the number of epidermal cell layers,a loosening of sponge tissue structure,and a decrease in vascular bundle cells in mutated expanded cassava.The results of this study provide theoretical guidance for further understanding the molecular mechanisms underlying the abnormal development of cassava leaf,as well as genetic resources and improvement strategies for cassava genetic improvement and germplasm innovation.关键词
木薯/转录组分析/突变/差异基因表达Key words
cassava/transcriptome analysis/mutation/differential gene expression引用本文复制引用
肖明昆,周迎春,沈正松,熊贤坤,罗鑫,白丽娜,刘光华,严炜,宋记明,张林辉,刘倩,段春芳,李月仙,姜太玲,沈绍斌..卷叶木薯及其突变体叶片的比较转录组分析[J].作物学报,2024,50(8):2143-2156,14.基金项目
本研究由兴滇英才支持计划项目——云南优良饲草品种选育及产业化关键技术集成示范和财政部和农业农村部国家现代农业产业技术体系建设专项(木薯-保山综合试验站,CARS-11-YNLGH)资助.This study was supported by the Xingdian Talent Support Plan Project:Integrated Demonstration of Key Technologies for the Breeding and Industrialization of Excellent Forage Varieties in Yunnan,and the China Agriculture Research System of MOF and MARA(Cassava-Comprehensive Experimental Station Project,CARS-11-YNLGH). （木薯-保山综合试验站,CARS-11-YNLGH）
