中国现代医生2024，Vol.62Issue(19)：1-7,7.DOI:10.3969/j.issn.1673-9701.2024.19.001
薇甘菊内酯衍生物LY19380b对人白血病细胞HEL的作用及机制研究
The effect and mechanism of a novel mikanolide derivative LY19380b on human leukemia cell HEL
摘要
Abstract
Objective To investigate the mechanism of mikanolide derivative LY19380b in inhibiting the growth of human leukemia cell HEL.Methods Methyl thiazolyl tetrazolium(MTT)assay was used to detect the effect of compound LY19380b on the proliferation of human tumor cells,flow cytometry was used to determine cell cycle,Annexin V-FITC/PI double staining and Hoechst 33258 staining were used to determine apoptosis.Western blot was used to analyze effects of LY19380b on cell cycle and apoptosis-related proteins.Results LY19380b had anti-proliferative activity on different human tumor cells,and significantly inhibited the growth of human leukemia cell HEL.It can also induce apoptosis and affect the cell cycle of HEL.In addition,LY19380b could significantly increase the expressions of P53,BIM,BID,BAD,BAX,FLIP,P21 and Cyclin B1 proteins,while decreased the expressions of Bcl-2,p-CDC25C,p-AKT,p-STAT3 and p-ERK proteins.Conclusion By increasing the expressions of pro-apoptotic proteins BIM,BID,BAD and BAX,decreasing the expression of anti-apoptotic protein Bcl-2,LY19380b induced the apoptosis of human leukemia cell HEL,and upregulated the expressions of P21 and Cyclin B1,thus leading to cell cycle arrest in HEL.关键词
白血病/薇甘菊内酯/凋亡/机制Key words
Leukemia/Mikanolide/Apoptosis/Mechanism分类
医药卫生引用本文复制引用
饶青,刘晟,黄磊,李艳梅,郑江..薇甘菊内酯衍生物LY19380b对人白血病细胞HEL的作用及机制研究[J].中国现代医生,2024,62(19):1-7,7.基金项目
贵州省自然科学基金资助项目(黔科合基础-ZK[2022]一般293) （黔科合基础-ZK[2022]一般293）
