中国现代医生2024，Vol.62Issue(19)：8-12,5.DOI:10.3969/j.issn.1673-9701.2024.19.002
超高效液相色谱质谱法快速测定血浆中6种蘑菇毒素
Rapid determination of six mushroom toxins in plasma by ultra performance liquid chromatography-mass spectrometry
摘要
Abstract
Objective To establish a method for rapid determination of isovelleral,α-amanitin,β-amanitin,γ-amanitin,dihydroxy-phallotoxin and carboxy-dihydroxy-phallotoxin in plasma by ultra performance liquid chromatography-mass spectrometry.Methods Take plasma samples and add acetonitrile solution in a 1:3 ratio.The sample was vortex mixed for 1 min,then centrifugated at 10 000r/min and 4℃ for 3min.The supernatant was injected,while 0.03％ammonia water and acetonitrile were used as mobile phases for ultra high performance liquid phase separation.Multi reaction monitoring was carried out in the positive ion mode of electric spray.Results The separation and detection of isovelleral,α-amanitin,β-amanitin,γ-amanitin,dihydroxy-phallotoxin and carboxyl-dihydroxy-phallotoxin in plasma could be achieved within 4 min by one injection.The linear range of six mushroom toxins in plasma was 40-500μg/L,and the detection limits were 10.0µg/L 10.0µg/L,10.0μg/L,6.0μg/L,6.0μg/L and 6.0μg/L(S/N=3),respectively.The relative standard deviations were 0.60％-1.90％(n=11),and the recovery rates were 90.8％-127.0％.Conclusion This method realizes the rapid and simultaneous determination of 6 kinds of mushroom toxins in plasma,including isovelleral.The operation is simple and the result is accurate,which provides a technical platform for the rapid confirmation of mushroom toxins in poisoned patients.关键词
蘑菇毒素/快速测定/血浆/超高效液相色谱质谱法Key words
Mushroom toxin/Rapid determination/Plasma/Ultra performance liquid chromatography-mass spectrometry分类
化学化工引用本文复制引用
李启,徐峻卿,钱佳清,纪律..超高效液相色谱质谱法快速测定血浆中6种蘑菇毒素[J].中国现代医生,2024,62(19):8-12,5.基金项目
浙江省医药卫生科技计划项目(2023KY1379) （2023KY1379）
丽水市科技计划项目(2022GYX37,2021ZDYF17) （2022GYX37,2021ZDYF17）
