植物营养与肥料学报2024，Vol.30Issue(7)：1387-1395,9.DOI:10.11674/zwyf.2024200
根际微生境中生化过程的原位可视化研究进展
Research progress in in situ visualization of biochemical processes at rhizosphere microhabitat
摘要
Abstract
Material exchange and energy flow are active in the rhizosphere and the adjacent area within several millimeters. The cycling of critical biogenic elements,including carbon,oxygen,and mineral elements in terrestrial ecosystems,predominantly occurs within this rhizosphere microhabitat. Visualizing the dynamic processes in the rhizosphere microhabitat can facilitate the capture and quantification of the process and function of material migration and transformation. Over the past few decades,two-dimensional imaging techniques have been developed for realizing in situ visualization. This article provides a systematic overview of the latest methods and research advancements in in situ visualization of rhizosphere chemicals,microbial environments,and metabolites,with an emphasis on the application prospects of multi-parameter imaging technology in studying the rhizosphere process. The future development direction and unsolved issues of in situ visualization of biochemical processes at rhizosphere microhabitat are also discussed. For more practical use of in situ visualization technology of rhizosphere in the researches of biochemical processes and mechanisms within microhabitats,researches should target on:1) developing straightforward,time-efficient,and cost-effective visualization technologies and performing compatibility assessments for multi-parameter and multi-method integration;2) advancing three-dimensional and four-dimensional visualization techniques for the rhizosphere,encompassing sample preparation and data synthesis,to investigate the spatio-temporal dynamics of the rhizosphere microhabitats;3) creating portable instrumentation to facilitate the field application of rhizosphere in situ visualization technology.关键词
根际微生境/多参数成像技术/原位可视化Key words
rhizosphere microhabitat/multi-parameter imaging technology/in situ visualization引用本文复制引用
顾文杰,刘冲,王丹,李雅莹,卢钰升,吴杭涛,解开治..根际微生境中生化过程的原位可视化研究进展[J].植物营养与肥料学报,2024,30(7):1387-1395,9.基金项目
广东省农业科学院农业资源与环境研究所科技条件平台创新项目(ZHS2023-01) （ZHS2023-01）
国家自然科学基金项目(42307410) （42307410）
广东省基础与应用基础研究基金项目(2024A1515011125) （2024A1515011125）
广东省重点领域研发计划项目(2023B0202010027) （2023B0202010027）
"十四五"广东省农业科技创新九大主攻方向"揭榜挂帅"项目(2023SDZG08) （2023SDZG08）
广东省科技计划项目(2021B1212050020) （2021B1212050020）
十四五新兴学科团队项目(202121TD). （202121TD）
