中国中西医结合杂志2024，Vol.44Issue(7)：847-855,9.DOI:10.7661/j.cjim.20240205.092
基于miR-1297/PTEN/PI3K/AKT通路探讨芪蓝方影响前列腺癌DU145细胞增殖及凋亡机制
Mechanism of Qilan Decoction's Effect on the Proliferation and Apoptosis of Prostate Cancer DU145 Cells Based on the miR-1297/PTEN/PI3K/AKT Signaling Pathway
摘要
Abstract
Objective To explore the possible mechanism of Qilan Decoction's effect on the proliferation and apoptosis of prostate cancer cells.Methods DU145 cells were divided into negative control group(NC mimics),Qilan Decoction group(NC mimics+QLF),miR-1297 over-expression group(miR-1297 mimics),miR-1297 over-expression+Qilan Decoction group(miR-1297 mimics+QLF),AKT activator group(SC79),AKT activator+Qilan Decoction group(SC79+QLF).CCK-8 method and EdU experiment were used to detect cell proliferation in each group.Flow cytometry were used to detect cell cycle and apoptosis in each group.RT-PCR was used to detect the mRNA expression of miR-1297,phosphatase and tensin homologue deleted on chromosome 10(PTEN),and protein kinase B(AKT).Western Blot was used to detect the expression of PTEN,phosphatidylinositol-3 kinase(PI3K),p-AKT,Cyclin D1,B-cell lymphoma/leukemia 2(Bcl-2),Bcl-2 related X protein(Bax),and Cleaved-caspase 3.Results Compared with negative control group,the activity,proliferation rate,EdU positive cell rate,G1 phase cell proportion,miRNA 1297 mRNA protein expression,PI3K,p-AKT,Cyclin D1,and Bcl-2 protein expression of DU145 cells in the Qilan Decoction group decreased,while the G2 phase cell proportion,Q1-LR(early apoptosis)+Q1-UR(late apoptosis),PTEN mRNA expression,PTEN,Bax,and Cleaved-caspase 3 protein expression increased(P＜0.01).The miR-1297 over-expression group and AKT activator group showed an increase in DU145 cell activity,proliferation rate,PI3K,p-AKT,Cyclin D1,and Bcl-2 protein expression,while the expression of Q1-LR+Q1-UR,PTEN proteins decreased(P＜0.01).Compared with the miR-1297 over-expression group,miR-1297 over-expression group+Qilan Decoction showed a decrease in DU145 cell activity,proliferation rate,G1 phase cell proportion,miRNA 1297 mRNA expression,PI3K,p-AKT,Cyclin D1,and Bcl-2 proteins,while the G2 phase cell proportion,Q1-LR+Q1-UR,PTEN mRNA expression,PTEN,Bax,and Cleaved-caspase 3 protein expression increased(P＜0.01).Compared with the AKT activator group,AKT activator+Qilan Decoction group showed a decrease in DU145 cell activity,proliferation rate,EdU positive cell rate,S phase cell proportion,PI3K,p-AKT,Cyclin D1,and Bcl-2 protein expression,while the G1 and G2 cell proportion,Q1-LR+Q1-UR,PTEN mRNA expression,PTEN,Bax,and Cleaved-caspase 3 protein expression increased(P＜0.01).Conclusion Qilan Decoction could inhibit the proliferation of DU 145 cells and promote the apoptosis of DU 145 cells by regulating the miR-1297/PTEN/PI3K/AKT signaling pathway.关键词
芪蓝方/前列腺癌/DU145细胞/增殖/凋亡/miR-1297/PTEN/PI3K/AKT通路/中药复方Key words
Qilan Decoction/prostate cancer/DU145 cells/proliferation/apoptosis/miR-1297/PTEN/PI3K/AKT signaling pathway/Chinese herbal compound引用本文复制引用
原凡,景明夷,周静,王家灏,常德贵,尤耀东..基于miR-1297/PTEN/PI3K/AKT通路探讨芪蓝方影响前列腺癌DU145细胞增殖及凋亡机制[J].中国中西医结合杂志,2024,44(7):847-855,9.基金项目
国家自然科学基金面上项目(No.81973866) （No.81973866）
四川省科技厅自然科学基金项目(No.2022NSFSC0684) （No.2022NSFSC0684）
四川省中医药管理局科学技术研究专项(No.2021ZD016) （No.2021ZD016）
成都中医药大学"杏林学者"学科人才科研提升计划研究专项(No.QNXZ2019020) （No.QNXZ2019020）
