中国中西医结合杂志2024，Vol.44Issue(7)：856-863,8.DOI:10.7661/j.cjim.20240522.103
仙甲汤抑制IL-6/JAK2/STAT3信号通路改善大鼠前列腺增生
Xianjia Decoction Improves Prostatic Hyperplasia in Rats Via Inhibiting IL-6/JAK2/STAT3 Signaling Pathway
摘要
Abstract
Objective To investigate the effect and mechanism of Xianjia Decoction(XD)on prostatic hyperplasia(BPH)in rats based on IL-6 mediated inflammatory response.Methods Totally 36 male SD rats were randomly divided into blank group,model group,XD high-dose,medium-dose,low-dose group and finasteride group,with 6 rats in each group.Except the blank group,each group was subcutaneously injected with testosterone propionate(5 mg/kg)for 30 consecutive days to prepare BPH model.After modeling,XD high,medium and low dose group were intragastric administration XD 27.18,13.59 and 6.80 g/kg,respectively,while finasteride group was intragastric administration finasteride 45 mg/kg,the blank group and model group were intragastric administration equal volume normal saline,the course was 30 consecutive days.Body weight,wet prostate weight and prostate volume of rats were measured and prostate index was calculated.HE staining was used to observe the histopathological changes of prostate.Mast cells of prostate tissue were observed and counted by toluidine blue staining.lnterleukin-6(IL-6)and interleukin-10(IL-10)were detected by ELISA.Janus kinase 2(JAK2),phosphorylated JAK2(p-JAK2),signal transducer and activator of transcription 3(STAT3)and phosphorylated STAT3(p-STAT3)were detected by Western Blot.The mRNA of IL-6,JAK2 and STAT3 in prostate tissue was determined by Real-time PCR.Results The glands in the prostate tissue of the blank group rats full and regular,while it is irregular,with proliferation of glandular epithelial cells,fibrous tissue proliferation in the matrix,and infiltration of inflammatory cells in the prostate gland of the model group rats.The intervention group showed varying degrees of improvement.Compared with the blank group,the prostate volume,wet weight,prostate index,mast cell number,IL-6 content,p-STAT3,p-JAK2 protein and IL-6,JAK2,STAT3 mRNA expression increased,and IL-10 content decreased in the model group(P＜0.01).Compared with the model group,the volume,wet weight,prostate index,the number of mast cells,the content of IL-6,p-STAT3 and p-JAK2 protein and IL-6,JAK2 and STAT3 mRNA expression decreased,and the content of IL-10 in prostate tissue was increased in the high and medium-dose XD group and finasteride group(P＜0.05,P＜0.01).The prostate volume,wet weight and STAT3 mRNA expression of rats in low dose XD group decreased(P＜0.05,P＜0.01).Compared with the finasteride group,the prostate volume,wet weight,index,mast cell number,IL-6 content,p-STAT3 and p-JAK2 protein expression decreased,and IL-10 content increased in the high-dose XD group(P＜0.05,P＜0.01).Conclusions XD can significantly improve BPH in rats,and its mechanism may be related to inhibiting the overactivation of IL-6/JAK2/STAT3 pathway.关键词
仙甲汤/前列腺增生/炎症反应/白细胞介素6/酪氨酸蛋白激酶2/转录激活因子3/中药复方Key words
Xianjia Decoction/prostatic hyperplasia/inflammatory response/interleukin-6/janus kinase 2/signal transducer and activator of transcription 3/Chinese herbal compound引用本文复制引用
刘祺,萧闵,江晓翠,蔚文垚,陈思易,姜兴宇,曹继刚..仙甲汤抑制IL-6/JAK2/STAT3信号通路改善大鼠前列腺增生[J].中国中西医结合杂志,2024,44(7):856-863,8.基金项目
国家自然科学基金青年项目(No.82104704) （No.82104704）
