中国现代中药2024，Vol.26Issue(7)：1157-1163,7.DOI:10.13313/j.issn.1673-4890.20240201001
基于UPLC特征图谱和含量测定的不同产地筋骨草质量评价
Quality Evaluation of Ajuga decumbens from Different Producing Areas Based on UPLC Characteristic Chromatogram and Content Determination
摘要
Abstract
Objective:To establish the characteristic chromatogram of Ajuga decumbens Thunb.by ultra-high performance liquid chromatography(UPLC)and method for determining the content of harpagoside and acetyl harpagoside.Methods:Waters BEH C18(150 mm×2.1 mm,1.7 μm)column was used.The mobile phase A was acetonitrile-methanol(4:1),and the mobile phase B was 0.2%phosphoric acid aqueous solution for gradient elution.The flow rate was 0.30 mL·min-1,and the column temperature was 30℃.The detection wavelength was 202 nm,and the injection volume was 2 μL.The characteristic chromatogram of A.decumbens was established to determine the content of harpagoside and acetyl harpagoside.Results:A total of 10 common fingerprint peaks were identified in the characteristic chromatograms of 17 batches of A.decumbens,among which four components were identified as harpagoside,acetyl harpagoside,luteonin-7-diglucosidic acid,and apigenin-7-diglucosidic acid.The mass fraction of harpagoside in the 17 batches of A.decumbens.was in the range of 0.33%-0.95%,while that of acetyl harpagoside was 0.47%-1.93%.Conclusion:The established UPLC characteristic chromatogram and the method for determining the content of harpagoside and acetyl harpagoside can provide a basis for the quality evaluation of A.decumbens.关键词
筋骨草/超高效液相色谱法/特征图谱/含量测定Key words
Ajuga decumbens Thunb./UPLC/characteristic chromatogram/content determination分类
医药卫生引用本文复制引用
丘娴,孙冬梅,周湘媛,罗佩琪,彭娇,谢远远,马懿飞,何民友,李振雨,陈向东..基于UPLC特征图谱和含量测定的不同产地筋骨草质量评价[J].中国现代中药,2024,26(7):1157-1163,7.基金项目
工业和信息化部2022年产业技术基础公共服务平台项目(2022-230-221) （2022-230-221）
佛山市南海区重点领域科技攻关专项(南科[2023]20号-18) （南科[2023]20号-18）
