中国现代中药2024，Vol.26Issue(7)：1173-1185,13.DOI:10.13313/j.issn.1673-4890.20240205001
基于UPLC-Q-Exactive Orbitrap MS和网络药理学研究银甲片治疗盆腔炎的有效成分及作用机制
Effective Components and Mechanism of Yinjia Tablets in Treating Pelvic Inflammatory Disease
摘要
Abstract
Objective:To study the effective components and mechanism of Yinjia Tablets in treating pelvic inflammatory disease.Methods:The chemical components of Yinjia Tablets were identified by ultra-performance liquid chromatography-quadrupole-Exactive Orbitrap-mass spectrometry(UPLC-Q-Exactive Orbitrap MS)combined with comparison with literature data and reference substances.The common targets shared by the chemical components and pelvic inflammatory disease were imported into String database to construct the protein-protein interaction(PPI)network.Gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed with DAVID.Cytoscape 3.9.1 was used to establish the"medicinal material-component-target-pathway"network.Molecular docking was performed in Maestro 11.9.Results:A total of 58 chemical components were identified in Yinjia Tablets,including flavonoids,organic acids,terpenoids,and alkaloids.Five core targets were obtained by the PPI network analysis,including steroid receptor coactivator(SRC),heat shock protein 90 alpha family class A member 1(HSP90AA1),phosphoinositide-3-kinase regulatory subunit 1(PIK3R1),signal transducers and activators of transcription 3(STAT3),and mitogen-activated protein kinase 3(MAPK3).The KEGG analysis results showed that Yinjia Tablets mainly exerted the therapeutic effect via phosphatidylinositol 3-kinase-protein kinase B(PI3K-Akt),MAPK and other pathways.Molecular docking results showed stable binding of luteoloside,kaempferol,luteolin,and diosmetin with five core targets including SRC,HSP90AA1,and PIK3R1.Conclusion:Yinjia Tablets may play a role in the treatment of pelvic inflammatory disease by regulating the PI3K-Akt and MAPK pathways via the active components such as luteoloside,kaempferol,luteolin,diosmetin,and apigenin.关键词
银甲片/盆腔炎/超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法/化学成分/作用机制Key words
Yinjia Tablets/pelvic inflammatory disease/UPLC-Q-Exactive Orbitrap MS/chemical component/mechanism分类
医药卫生引用本文复制引用
黄雪梅,丁银,苟晓玲,熊双凤,宗毅,唐策,范刚,黄叶芳..基于UPLC-Q-Exactive Orbitrap MS和网络药理学研究银甲片治疗盆腔炎的有效成分及作用机制[J].中国现代中药,2024,26(7):1173-1185,13.基金项目
国家自然科学基金青年科学基金项目(82205175) （82205175）
四川省科技计划项目(2022YFS0415) （2022YFS0415）
