中国现代中药2024，Vol.26Issue(7)：1208-1218,11.DOI:10.13313/j.issn.1673-4890.20231201005
基于Meta和网络药理学分析参苓白术散治疗糖尿病肥胖的作用机制
Mechanism of Shenling Baizhu Powder in Treating Obesity Associated with Diabetes Based on Meta-analysis and Network Pharmacology
摘要
Abstract
Objective:To evaluate the therapeutic effect of Shenling Baizhu Powder on the obesity associated with diabetes by Meta-analysis and decipher the treatment mechanism by combining network pharmacology and animal experiments.Methods:The relevant publications were included in this study.The body mass index(BMI),glycosylated hemoglobin(HbA1c),2 h postprandial blood glucose(2 h PG),fasting blood glucose(FBG),Homeostatic Model Assessment for Insulin Resistance(Home-IR),and waist-to-hip ratio were used as outcome indicators to evaluate the therapeutic effect of Shenling Baizhu Powder on the obesity associated with diabetes.The genes related to this disease were downloaded from the Gene Expression Omnibus(GEO)database,and the targets of Shenling Baizhu Powder were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Traditional Chinese Medicine Integrated Database(TCMID),and Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine(BATMAN-TCM).The protein-protein interactions of the common targets shared by the disease and the drug were analyzed,and then the core target proteins were screened out by the topological analysis.A mouse model of obesity associated with diabetes was established.After grouping,the mice were treated with Shenling Baizhu Powder by gavage.After two weeks,the body weight,blood glucose level,and Lee's index were recorded.The volume changes of adipocytes in each group of mice were observed by hematoxylin-eosin staining,and the levels of cholesterol,triglycerides,and core target proteins in the mouse serum were measured.Result:A total of 9 articles were included,with complete data and no publication bias.The evaluation indicated a low risk.After treatment with Shenling Baizhu Powder,the BMI,HbA1c,2 h PG,Home-IR,and waist-to-hip ratio of the patients were recovered to some extent.There were 59 common targets shared by Shenling Baizhu Powder and obesity associated with diabetes,and the core targets were apolipoprotein E(APO-E),insulin growth factor-1(IGF-1),and plasminogen activator inhibitor-1(PAI-1).Shenling Baizhu Powder mainly regulated the biological processes such as cellular response to oleic acid(GO:0071400),positive regulation of transforming growth factor beta 1 production(GO:0032914),and negative regulation of plasminogen activation(GO:0010757).It modulated the signaling pathways such as unsaturated fatty acid biosynthesis(hsa01040)and fatty acid metabolism(hsa01212)to treat obesity associated with diabetes.The results of animal experiments showed that the treatment with Shenling Baizhu Powder by gavage did not cause significant change in blood glucose level,decreased the body weight and Lee's index,reduced the diameter of adipocytes,and lowered the levels of cholesterol,triglycerides,APO-E,IGF-1,and PAI-1.Conclusion:The results of Meta-analysis and animal experiments suggest that Shenling Baizhu Powder has obvious therapeutic effect on the obesity associated with diabetes.Shenling Baizhu Powder may exert the therapeutic effect by regulating the synthesis of unsaturated fatty acids and fatty acids,reducing the transformation of sugar and fat,correcting the imbalance of energy metabolism,and modulating autophagy to promote the decomposition of the adipose tissue.关键词
参苓白术散/糖尿病肥胖/作用机制/荟萃分析法/网络药理学Key words
Shenling Baizhu Powder/obesity associated with diabetes/mechanism/Meta-analysis/network pharmacology分类
医药卫生引用本文复制引用
付芳,刘莉娜,章新友,吴地尧,闵捷..基于Meta和网络药理学分析参苓白术散治疗糖尿病肥胖的作用机制[J].中国现代中药,2024,26(7):1208-1218,11.基金项目
江西中医药大学博士自然科学研究项目(2020BSZR017) （2020BSZR017）
江西省中医药中青年骨干人才培养计划项目(第三批) （第三批）
江西中医药管理局癌病方证信息数据挖掘重点研究室项目(ZDYJS202202) （ZDYJS202202）
