中国现代中药2024，Vol.26Issue(7)：1219-1226,8.DOI:10.13313/j.issn.1673-4890.20231130002
响应面法优化天仙果总黄酮提取工艺及抗氧化活性研究
Optimization of the Extraction Process of Total Flavonoids from Ficus erecta by Response Surface Methodology and Its Antioxidant Activity
摘要
Abstract
Objective:To optimize the extraction process and determine the antioxidant activity of total flavonoids from Ficus erecta(Thunb.)var.beecheyana(Hook.et Arn.)King.Methods:Four factors,namely ethanol concentration,material-liquid ratio,extraction time,and number of extractions,were selected to obtain the optimal conditions through response surface methodology and mathematical modeling,thus improving the extraction rate of total flavonoids from F.erecta.The in vitro antioxidant activity was investigated,measuring 1,1-Diphenyl-2-picrylhydrazyl radical(DPPH),2,2-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS),and hydroxyl radical scavenging capacities.Results:The optimal extraction process for total flavonoids from F.erecta was identified with 60%ethanol,1:23 material-liquid ratio,and 18-min extraction time,under which the extraction rate was 16.25 mg·g-1.The scavenging rates of total flavonoids from F.erecta for DPPH,ABTS,and hydroxyl radicals reached 92.84%,89.34%,and 69.73%,respectively.Conclusion:The optimized extraction process was operable,stable,and reliable,exhibiting good antioxidant activity.关键词
天仙果/总黄酮/提取工艺/响应面法/抗氧化活性Key words
Ficus erecta(Thunb.)var.beecheyana(Hook.et Arn.)King/total flavonoids/extraction process/response surface methodology/antioxidant activity分类
医药卫生引用本文复制引用
黄雅瑜,林米妮,蔡树芸,杨光乐,张秋萍,张怡评..响应面法优化天仙果总黄酮提取工艺及抗氧化活性研究[J].中国现代中药,2024,26(7):1219-1226,8.基金项目
福建省科技计划项目(2021N0029) （2021N0029）
厦门市科技计划项目(3502Z20227107) （3502Z20227107）
