中医药学报2024，Vol.52Issue(8)：17-21,5.DOI:10.19664/j.cnki.1002-2392.240153
附子理中汤对非酒精性脂肪肝模型大鼠的保护作用及机制研究
Protective Effect and Mechanism of Fuzi Lizhong Decoction on Nonalcoholic Fatty Liver Model Rats
摘要
Abstract
Objective:To explore the protective effect of Fuzi Lizhong decoction(FLD)on non-alcoholic fatty liver disease(NAFLD)model rats and its possible mechanism.Methods:NAFLD model rats were established by gavage with fat milk,and treated with FLD and positive control drug polyene phosphatidylcholine capsule.HE and oil red O staining were used to observe the pathological damage and fat deposition of liver and ileum.The levels of serum LDL-C,HDL-C,AST and ALT were detected by automatic biochemical analyzer.The levels of TNF-α and NF-κBp65 genes in ileum tissue were detected by qRT-PCR,and the levels of serum TNF-α protein were detected by ELISA.Results:Compared with the control group,the liver and ileum of the model group suffered from pathological damage and fat deposition,and the levels of LDL-C,AST,ALT,TNF-α and NF-κBp65 all increased significantly(P<0.05),while the level of HDL-C decreased significantly(P<0.05).Compared with the model group,the fat deposition in liver and ileum of rats in polyene phosphatidylcholine capsule and FLD groups decreased,and the levels of LDL-C,AST,ALT,TNF-α and NF-κBp65 decreased significantly(P<0.05),while the level of HDL-C increased significantly(P<0.05).Conclusion:Fuzi Lizhong decoction can reduce the pathological damage and fat deposition of liver and ileum in NAFLD rats by inhibiting TNF-α/NF-κB signaling pathway.关键词
附子理中汤/非酒精性脂肪肝病/TNF-α/NF-κB信号通路Key words
Fuzi Lizhong decoction/Nonalcoholic fatty liver disease/TNF-α/NF-κB signaling pathway分类
医药卫生引用本文复制引用
杜念龙,杨家耀,徐丹,张书,时昭红..附子理中汤对非酒精性脂肪肝模型大鼠的保护作用及机制研究[J].中医药学报,2024,52(8):17-21,5.基金项目
武汉市卫健委医学科研项目(WZ21Q12) （WZ21Q12）
