附子理中汤对非酒精性脂肪肝模型大鼠的保护作用及机制研究OACSTPCD

Objective:To explore the protective effect of Fuzi Lizhong decoction(FLD)on non-alcoholic fatty liver disease(NAFLD)model rats and its possible mechanism.Methods:NAFLD model rats were established by gavage with fat milk,and treated with FLD and positive control drug polyene phosphatidylcholine capsule.HE and oil red O staining were used to observe the pathological damage and fat deposition of liver and ileum.The levels of serum LDL-C,HDL-C,AST and ALT were detected by automatic biochemical analyzer.The levels of TNF-α and NF-κBp65 genes in ileum tissue were detected by qRT-PCR,and the levels of serum TNF-α protein were detected by ELISA.Results:Compared with the control group,the liver and ileum of the model group suffered from pathological damage and fat deposition,and the levels of LDL-C,AST,ALT,TNF-α and NF-κBp65 all increased significantly(P<0.05),while the level of HDL-C decreased significantly(P<0.05).Compared with the model group,the fat deposition in liver and ileum of rats in polyene phosphatidylcholine capsule and FLD groups decreased,and the levels of LDL-C,AST,ALT,TNF-α and NF-κBp65 decreased significantly(P<0.05),while the level of HDL-C increased significantly(P<0.05).Conclusion:Fuzi Lizhong decoction can reduce the pathological damage and fat deposition of liver and ileum in NAFLD rats by inhibiting TNF-α/NF-κB signaling pathway.