祛毒汤的抗菌抗炎活性及其作用机制研究OACSTPCD

Objective:To explore the antibacterial and anti-inflammatory effects of Qudu decoction against clinical pathogens,providing an experimental basis for its use in treating infections with traditional Chinese medicine.Methods:Various standard strains,quality control strains,and common clinical pathogens were selected to assess the antimicrobial activities of Qudu decoction using the Oxford cup diffusion method and agar dilution method,determining the zones of inhibition and minimum inhibitory concentrations.The growth rates of strains and agar plate colony counts were used to evaluate the bacteriostatic and bactericidal effects of Qudu decoction fumigation.An inflammatory cell model of RAW264.7 was established using LPS and bacterial lysate.Cell viability was assessed by the MTT assay,and the anti-inflammatory effects were evaluated by measuring the concentrations of nitric oxide(NO),tumor necrosis factor-alpha(TNF-α),and interleukin-6(IL-6)in the cell culture supernatants.Results:1.Qudu decoction exhibited strong antimicrobial activity against Candida and showed varying degrees of antibacterial effects against different bacterial strains.2.The antifungal activity of Qudu decoction against Candida was not significantly related to the resistance profiles of existing antifungal drugs,while its antibacterial effects correlated with specific resistant bacteria.3.Qudu decoction effectively inhibited the secretion of NO,TNF-α,and IL-6 in RAW264.7 cells induced by LPS and bacterial lysate.Conclusion:Qudu decoction possesses a specific antimicrobial spectrum,being most effective against Candida.The mechanism of its antifungal activity may differ from the resistance mechanisms of existing antibiotics,and its anti-inflammatory mechanism might be related to the inhibition of TNF-α and IL-6 secretion and the reduction of NO production.