通消阳和颗粒联合环磷酰胺对4T1乳腺癌荷瘤小鼠移植瘤的影响OACSTPCD

Objective:To observe the effects of Tongxiaoyanghe granules combined with cyclophosphamide on 4T1 breast cancer-bearing mice.Methods:Female BALB/c mice were used to establish a 4T1 breast cancer tumor model,randomly divided into a model group,Tongxiaoyanghe granules group(granules group),Tongxiaoyanghe granules+cyclophosphamide group(combined group),and a cyclophosphamide group,with eight mice in each group.An additional eight mice were selected as the control group.The tumor growth of mice in each group was monitored,and the tumor growth-time curves were plotted.Tumor inhibition rates were calculated.Peripheral blood white blood cell counts and the proportions of neutrophils,lymphocytes,and monocytes were measured,and biochemical indicators of liver and kidney functions were determined.Spleen immune cell numbers and the proportions of CD4+and CD8+T cells were quantified,and cells from the lungs,brain,liver,and peripheral blood of mice were cultured to assess the distant organ metastasis of tumor cells.Results:Compared with the model group,the tumor growth rate was slowest in the combined group(P<0.01),and the tumor inhibition rate was the highest(P<0.01).The white blood cell count was significantly reduced(P<0.01).Compared with the cyclophosphamide group,the proportion of lymphocytes in the combined group increased significantly(P<0.01),while the proportions of neutrophils and monocytes decreased significantly(P<0.01).The liver and kidney function indicators were normal,showing no early damage.There was a significant increase in T and B lymphocytes,a decrease in MDSCs cells,and an increase in the ratio of CD4+to CD8+T cells(P<0.05).No distant organ metastasis of breast cancer cells was observed.Conclusion:Tongxiaoyanghe granules combined with cyclophosphamide significantly inhibit the growth of transplanted tumors in breast cancer-bearing mice,and Tongxiaoyanghe granules can reduce the liver and kidney damage and the decrease in immune function caused by cyclophosphamide.The combination can significantly enhance the immune function of breast cancer mice and inhibit the distant metastasis of tumor cells.