共载吲哚菁绿与苦参碱温敏水凝胶的制备与表征OACSTPCD

Objective:To prepare a kind of thermosensitive hydrogel that can be used for intratumoral injection.In this study,a thermosensitive hydrogel(MAT-ICG-gel)co-loaded with indocyanine green(ICG)and matrine(MAT)was designed using ICG as photosensitizer and poloxamer F127 and F68 as gel matrix.And the physicochemical properties of MAT-ICG-gel were characterized.Methods:The cold solution method was adopted to select the optimal preparation process of MAT-ICG-gel through single factor experiment,with the gelation temperature and gelation time as the evaluation indexes.The morphology,rheological properties,photothermal conversion properties,dissolution properties,in vitro release and preliminary stability of MAT-ICG-gel prepared by using the optimal preparation technology were investigated.Results:The optimal prescription composition of MAT-ICG-GEL was F127∶F68=20∶3.5,MAT∶ICG=10∶1.The optimal formulation was used to prepare MAT-ICG gel,with a gelation temperature of(37.3±0.2)℃,a gelation time of(181±3.6)s,and good stability within 7 days.The viscosity of MAT-ICG-gel increased sharply from 37℃and became stable at 42℃.MAT-ICG-gel exhibited good photothermal conversion performance under 808 nm of laser irradiation.When compared to 37℃,the dissolution rate and drug release rate of MAT-ICG-gel at 42℃were both more slowly.Conclusion:The preparation process of MAT-ICG-gel is simple and feasible.MAT-ICG-gel has good photothermal conversion performance,biodegradability and temperature-dependent sustained drug release characteristics.This study can provide new ideas and theoretical basis for the development of new dosage forms of matrine combined with photothermal therapy.