中医药信息2024，Vol.41Issue(7)：7-12,6.DOI:10.19656/j.cnki.1002-2406.20240702
基于多因素造模法改良脾胃气虚型糖尿病胃轻瘫大鼠模型制备方法研究
Study on the Method of Improving the Model Preparation of Diabetes Gastroparesis Rats with Spleen and Stomach Qi Deficiency Based on Multi Factor Modeling
摘要
Abstract
Objective:To establish and evaluate the model of diabetes gastroparesis(DGP)rats with spleen and stomach qi deficiency by multifactor modeling combined with streptozotocin(STZ)and high sugar and high-fat diet.Methods:Twenty SPF grade Wistar rats were randomly divided into a blank group(n=8)and a modeling group of(n=12).The spleen stomach qi deficiency rat model was replicated using a multifactor modeling method,and an evaluation scale for the spleen stomach qi deficiency model was developed.Rats that met the criteria were included in the experiment.The DGP rat model was replicated by injecting small doses of STZ multiple times combined with high sugar and high fat diet,and samples were collected after 6 weeks of experiment commencement.Blood glucose,body mass,gastric emptying rate,and small intestine propulsion rate of rats were randomly observed.Elisa method was used to detect the levels of serum amyloid protein(SAA),D-xylose,and lactate dehydrogenase(LDH)in serum.The pathological structural changes of the spleen were observed under light microscopy.Results:Compared with the rats in blank group,the rats in model group had poorer general conditions(in mental state,defecation,and dietary intake),and decreased body mass,with a statistically significant difference(P<0.05).The random blood glucose levels in the modeling group significantly increased(P<0.01).Compared with the blank group,the gastric emptying rate,small intestine propulsion rate,SAA,D-xylose,and LDH levels in the modeling group decreased significantly(P<0.05,P<0.01).Under light microscopy,it was observed that the splenic capsule of the model group was thickened,the boundary between red and white pulp was unclear,and lymphocytes were reduced.Evaluation of DGP syndrome type of spleen and stomach qi deficiency showed that the model group rats showed symptoms of spleen and stomach qi deficiency,such as fatigue,decreased body mass,curling up,poor mobility,squinting eyes,dull fur color,and dark tongue color.Conclusion:The rat model established by the multi factor modeling method meets the standard of DGP rats with spleen stomach qi deficiency.关键词
糖尿病胃轻瘫/脾胃气虚/模型制备/多因素造模法Key words
Diabetic gastroparesis/Spleen and stomach qi deficiency/Model preparation/Multifactor modeling method引用本文复制引用
魏昭晖,李荣科,张磊,马欣欣,郭倩,刘苗,万生芳..基于多因素造模法改良脾胃气虚型糖尿病胃轻瘫大鼠模型制备方法研究[J].中医药信息,2024,41(7):7-12,6.基金项目
国家自然科学基金项目(82060914) （82060914）
甘肃省优秀博士生项目(23JRRA1225) （23JRRA1225）
甘肃中医药大学研究生创新项目(2022-07) （2022-07）
