感染、炎症、修复2023，Vol.24Issue(3)：173-179,封2,封3,9.DOI:10.3969/j.issn.1672-8521.2023.03.005
蛋白酶活化受体1在热打击人脐静脉内皮细胞炎症激活中作用的研究
Study on the role of protease-activated receptor 1 in the inflammatory activation of human umbilical vein endothelial cells by heat stress
摘要
Abstract
Objective:To observe mRNA and protein expression level of protease-activated receptor 1(PAR1),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),intercellula cell adhesion molecule-1(ICAM-1)after heat sress(HS)of human umbilical vein endothelial cells(HUVECs),and to study the role of PAR1 in inflammtory responses of HUVECs by heat stress.Methods:HUVECs were pretreated with PAR1 inhibitor SCH79797(SCH),PAR1 agonist TFLLR-NH2(TFL),PRA1siRNA and PAR1 adenovirus respectively and then reeeived HS(42℃2 h),different groups were extracted to detect mRNA and protein expression of PAR1,TNF-α,IL-6,and ICAM-1.Mononuclear cells adhesion to HUVECs was observed by fluorescence microscopy after pre-treated with PAR1 inhibitor,agonist,siRNA and PAR1 adenovirus respectively in HUVECs in 8 hours after heat stress.Results:Control with normal group,mRNA and protein expressions of PAR1,TNF-α,IL-6 and ICAM-1 increased after heat stress(P＜0.05,P＜0.01);PAR1 protein expression of HUVECs after high normal temperature culture and heat stress could be effectively decreased by siRNA transfection and increased by adenovirus transfection(P＜0.01).Control with HS group,inhibiting the expression of PAR1 or PRA1siRNA lead to TNF-α,IL-6,and ICAM-1 mRNA and pro-tein level decreasing and the adhesion of monocytes decreasing(P＜0.05,P＜0.01);otherwise,increasing PAR1 expression or AdPAR1 lead to TNF-α,IL-6,and ICAM-1 mRNA and protein level increasing and the adhesion increasing(P＜0.05,P＜0.01).Conclusions:HS can lead to PAR1 activation in HUVECs,which leads to the activation of inflammatory pathways in HUVECs.关键词
热打击/人脐静脉内皮细胞/蛋白酶活化受体1/炎症反应/单核细胞Key words
Heat stress/Human umbilical vein endothelial cell/protease-activated receptor 1/Inflammatory responses/Monocyte分类
医药卫生引用本文复制引用
王郑莲,徐秋林,邓茂林,刘亚楠,苏磊..蛋白酶活化受体1在热打击人脐静脉内皮细胞炎症激活中作用的研究[J].感染、炎症、修复,2023,24(3):173-179,封2,封3,9.基金项目
广东省自然科学基金博士启动项目(S2013040015661) （S2013040015661）
中国博士后科学基金项目(2014M552180) （2014M552180）
