微小RNA-34a沉默信息调节因子1与急性缺血性脑卒中患者侧支循环的关系OACSTPCD
Relationship between miR-34a,sirt1 and collateral circulation in patients with acute ischemic stroke
目的 分析微小RNA-34a(miR-34a)、沉默信息调节因子1(sirt1)在急性缺血性脑卒中患者侧支循环建立中的预测价值.方法 纳入急性缺血性脑卒中患者82例为研究对象,记录患者入院NIHSS评分等基本资料,根据入院5d后DSA结果分为侧支循环不良组(32例)和侧支循环良好组(50例).采用酶联免疫吸附法(ELISA)检测血清sirt1水平,实时荧光定量PCR检测血清miR-34a水平.采用Pearson法进行sirt1、miR-34a与一般资料的相关性分析,ROC曲线评价sirt1、miR-34a对侧支循环建立的预测价值.采用Logistic回归分析影响侧支循环建立的因素.结果 侧支循环不良组高血压(65.63%)、高胆固醇血症(28.13%)、入院NIHSS评分[(16.57±3.30)分]、脑梗死体积[(11.02±3.27)cm3]、miR-34a(1.58±0.42)均高于侧支循环良好组[40.00%、8.00%、(10.14±3.01)分、(6.39±2.01)cm3,(1.00±0.23)],sirt1[(5.09±1.57)μg/L]低于侧支循环良好组[(7.63±1.53)μg/L,P<0.05].侧支循环不良急性缺血性脑卒中患者miR-34a与sirt1水平均呈负相关(r=-0.521、-0.529,P<0.05),高血压、高胆固醇血症、入院NIHSS评分、脑梗死体积与miR-34a均呈正相关(P<0.05),与sirt1均呈负相关(P<0.05).miR-34a、sirt1及其联合预测急性缺血性脑卒中患者侧支循环建立不良的曲线下面积(AUC)分别为0.882、0.901、0.935.miR-34a、sirt1是急性缺血性脑卒中患者侧支循环建立的独立影响因素(P<0.05).侧支循环不良组预后不良患者比例高于侧支循环良好组(P<0.05).结论 miR-34a、sirt1对急性缺血性脑卒中患者侧支循环建立有一定的预测作用.
Objective To analyze the predictive value of microRNA-34a(miR-34a)and silent information regulator 1(sirt1)in the establishment of collateral circulation in patients with acute ischemic stroke.Methods Eighty-two patients with acute ischemic stroke were enrolled as the research objects,and the basic data such as NIHSS score of the patients were recorded,and were divided into the poor collateral circulation group(32 cases)and the good collateral circulation group(50 cases)according to digital subtraction angiography 5 days after admission.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum sirt1 level,and real-time fluorescent quantitative PCR was used to detect serum miR-34a level.Pearson method was used to analyze the correlation between sirt1,miR-34a and general data.ROC curve was used to evaluate the predictive value of sirt1 and miR-34a on the establishment of collateral circulation.Logistic regression was used to analyze the factors affecting the establishment of collateral circulation.Results Hypertension(65.63%),hypercholesterolemia(28.13%),NIHSS score at admission((16.57±3.30)points),cerebral infarction volume((11.02±3.27)cm3),and miR-34a(1.58±0.42)in the poor collateral circulation group were higher than those in the good collateral circulation group(40.00%,8.00%,(10.14±3.01)points,(6.39±2.01)cm3,1.00±0.23,respectively),while sirt1((5.09±1.57)μg/L)was lower than that in the good collateral circulation group((7.63±1.53)μg/L,P<0.05).The levels of miR-34a and sirt1 were negatively correlated in acute ischemic stroke patients with poor collateral circulation(r=-0.521,-0.529,P<0.05),the hypertension,hypercholesterolemia,NIHSS score at admission,and cerebral infarction volume were positively correlated with miR-34a(P<0.05),and were negatively correlated with sirt1(P<0.05).The area under the curve(AUC)of miR-34a,sirt1,and the combination for predicting poor establishment of collateral circulation in patients with acute ischemic stroke was 0.882,0.901,and 0.935,respectively.miR-34a and sirt1 were independent factors influencing the establishment of collateral circulation in patients with acute ischemic stroke(P<0.05).The proportion of patients with poor prognosis in the group with poor collateral circulation was higher than that in the group with good collateral circulation(P<0.05).Conclusion miR-34a and sirt1 have certain predictive effects on the establishment of collateral circulation in patients with acute ischemic stroke.
张敏;王敏;司君增
山东第一医科大学附属人民医院,山东 济南 271199
临床医学
急性缺血性脑卒中侧支循环微小RNA-34a沉默信息调节因子1
Acute ischemic strokeCollateral circulationmicroRNA-34aSilent information regulator 1
《中国实用神经疾病杂志》 2024 (008)
931-935 / 5
济南市卫生健康委员会科技计划项目(编号:2021-2-18)
评论