计量学报2024，Vol.45Issue(7)：1081-1088,8.DOI:10.3969/j.issn.1000-1158.2024.07.19
基于UPLC-DAD-CAD双检测器串联的DON纯度测量方法对比研究
Comparative Study on DON Purity Analysis Based on UPLC with DAD Coupled to CAD
摘要
Abstract
An ultra-high performance liquid chromatography with diode array detector coupled to charged aerosol detector(UPLC-DAD/CAD)method was established for comparative study on deoxynivalenol(DON)purity analysis.The optimized chromatographic condition was isocratic elution with 6%methanol for 20 minutes.There were obvious differences in the types and amounts of impurities detected by the two detectors.17 impurities were identified by DAD with impurity 15 accounting for the highest proportion(0.847%),following by impurity 17(0.0812%)and impurities 2,5 and 8(above 0.01%).9 impurities were identified by CAD with impurity 17 accounting for the highest proportion(0.406 0%),following by impurity 15(0.3499%)and impurities 10,11 and 12(above 0.1%).The purity obtained by DAD detection method was 98.92%,significantly higher than that obtained by CAD detection method(98.61,p<0.001).And the relativestandarddeviationof DADmethod(0.00670%)wasalso lower than that of the CAD method(0.046 1%).The measurementuncertainty of purity by the DAD method was 0.463%,mainly introduced by response differences of the impurities;while it was 0.050 3%for the CAD method,which was mainly introduced by measurement repeatability.The results may be helpful for the chromatographic purity analysis of DON.关键词
生物计量/脱氧雪腐镰刀菌烯醇/C/DAD/CAD/不确定度Key words
biometrology/deoxynivalenol/chromatographic purity analysis/DAD/CAD/uncertainty分类
通用工业技术引用本文复制引用
王松山,雷达,贾睿晖,李丽,周明慧,王松雪..基于UPLC-DAD-CAD双检测器串联的DON纯度测量方法对比研究[J].计量学报,2024,45(7):1081-1088,8.基金项目
国家重点研发计划(2022YFF0710400) （2022YFF0710400）
