3D打印明胶/骨粉支架促成骨分化的体外研究OACSTPCD
In vitro study of 3D printed gelatin/bone powder scaffold promoting osteogenesis differentiation
目的:将甲基丙烯酸酰化明胶(gelatin methacryloyl,GelMA)与Bio-Oss骨粉制备成复合生物墨水.通过生物 3D打印构建Bio-Oss/GelMA载骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)复合骨组织工程支架,检测其生物相容性以及促体外成骨分化能力.方法:在GelMA中添加不同浓度的Bio-Oss,利用 3D生物打印制备GelMA/Bio-Oss/BMSCs复合支架.通过电镜观察、活/死细胞染色、细胞增殖实验、碱性磷酸酶(ALP)染色,RT-qPCR检测,探讨GelMA/Bio-Oss/BMSCs生物支架最适合的浓度.结果:骨组织工程支架结构稳定.添加Bio-Oss对打印过程中和打印后体外培养过程中细胞存活率没有明显影响.高浓度Bio-Oss组显著增强成骨基因OPN、RUNX2、OCN 的表达和 ALP 的活性.结论:GelMA/Bio-Oss/BMSCs 复合支架生物相容性好且具有明显促BMSCs成骨分化作用.
Objective:To prepare a composite bioink from methylacry lated gelatin(GelMA)and Bio-Oss.The Bio-Oss/GelMA-loaded bone marrow mesenchymal stem cell(BMSCs)composite bone tissue engineering scaffold was constructed by 3D bioprinting to explore its biocompatibility and in vitro osteogenic differentiation ability.Methods:GelMA/Bio-Oss/BMSCs were prepared by 3D bioprinting by adding different concentrations of Bio-Oss in GelMA.The best suitable concentra-tion of GelMA/Bio-Oss/BMSCs was determined by electron microscopy,live/dead staining,cell proliferation experiments,al-kaline phosphatase(ALP)staining,and RT-qPCR.Results:Bone tissue engineering scaffold structure was stable.The added of Bio-Oss showed no obvious toxicity on cell viability culture both during and after printing.High concentration Bio-Oss group promoted the expression of osteogenic genes OPN,RUNX2,OCN and enhanced ALP activity significantly.Conclusion:GelMA/Bio-Oss/BMSCs composite scaffolds has good biocompatibility and has an obvious effect in promoting the osteogenic differentiation of BMSCs.
李昕;刘蓉;刘巧;贺昊;谢雨朦;李哲争;朱丽雷
湖南中医药大学研究生院 湖南 长沙 410208长沙市口腔医院牙周黏膜科 湖南 长沙 410000
口腔医学
3D生物打印甲基丙烯酰化明胶骨髓间充质干细胞成骨分化骨再生骨缺损
3D bioprintingGelatin methacryloylBone marrow mesenchymal stem cellsOsteogenesis differentia-tionBone regenerationBone defect
《临床口腔医学杂志》 2024 (008)
456-460 / 5
湖南中医药大学校级科研基金项目(2022XYLH130);长沙市自然科学基金项目(kq2208486)
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