临床口腔医学杂志2024，Vol.40Issue(8)：456-460,5.DOI:10.3969/j.issn.1003-1634.2024.08.003
3D打印明胶/骨粉支架促成骨分化的体外研究
In vitro study of 3D printed gelatin/bone powder scaffold promoting osteogenesis differentiation
摘要
Abstract
Objective:To prepare a composite bioink from methylacry lated gelatin(GelMA)and Bio-Oss.The Bio-Oss/GelMA-loaded bone marrow mesenchymal stem cell(BMSCs)composite bone tissue engineering scaffold was constructed by 3D bioprinting to explore its biocompatibility and in vitro osteogenic differentiation ability.Methods:GelMA/Bio-Oss/BMSCs were prepared by 3D bioprinting by adding different concentrations of Bio-Oss in GelMA.The best suitable concentra-tion of GelMA/Bio-Oss/BMSCs was determined by electron microscopy,live/dead staining,cell proliferation experiments,al-kaline phosphatase(ALP)staining,and RT-qPCR.Results:Bone tissue engineering scaffold structure was stable.The added of Bio-Oss showed no obvious toxicity on cell viability culture both during and after printing.High concentration Bio-Oss group promoted the expression of osteogenic genes OPN,RUNX2,OCN and enhanced ALP activity significantly.Conclusion:GelMA/Bio-Oss/BMSCs composite scaffolds has good biocompatibility and has an obvious effect in promoting the osteogenic differentiation of BMSCs.关键词
3D生物打印/甲基丙烯酰化明胶/骨髓间充质干细胞/成骨分化/骨再生/骨缺损Key words
3D bioprinting/Gelatin methacryloyl/Bone marrow mesenchymal stem cells/Osteogenesis differentia-tion/Bone regeneration/Bone defect分类
医药卫生引用本文复制引用
李昕,刘蓉,刘巧,贺昊,谢雨朦,李哲争,朱丽雷..3D打印明胶/骨粉支架促成骨分化的体外研究[J].临床口腔医学杂志,2024,40(8):456-460,5.基金项目
湖南中医药大学校级科研基金项目(2022XYLH130) （2022XYLH130）
长沙市自然科学基金项目(kq2208486) （kq2208486）
