临床神经外科杂志2024，Vol.21Issue(4)：371-376,6.DOI:10.3969/j.issn.1672-7770.2024.04.003
基于p38 MAPK/NF-κB信号通路探究芍药醇对创伤性脑损伤小鼠神经保护作用的机制研究
Mechanism of neuroprotective effect of paeoniflorol on traumatic brain injury mice based on p38 MAPK/NF-κB signaling pathway
摘要
Abstract
Objective To predict the effect of paeoniflorol on neurological function recovery and neuroinflammation after traumatic brain injury(TBI)and evaluate its potential value in clinical practice.Methods A model of TBI in adult mice was established by controlled cortical impingement(CCI)method,and the therapeutic efficacy of paeoniflorol was evaluated after tail vein injection.Network pharmacology predicted that p38 MAPK/NF-κB was a potential route for the treatment of TBI with paeoniflorol.Western blotting and enzyme-linked immunosorbent assay were used to evaluate the expression of target proteins and inflammatory cytokines.Nissl staining was used to observe neuronal damage.Modified Neurological Severity Score(mNSS)was used to evaluate neurological recovery after TBI.Results Paeoniflorol administration after TBI prevented neuronal death,reduced neuroinflammation and promoted neurological recovery in CCI mice.Network pharmacology studies had shown that paeoniflorol may reflect its therapeutic efficacy through the p38 MAPK/NF-κB pathway.Conclusions Paeoniflorol has superior neuroprotective and anti-inflammatory properties during the course of TBI disease development,and its mechanism may be related to the regulation of the p38 MAPK/NF-κB signaling pathway.关键词
芍药醇/创伤性脑损伤/神经功能/炎症Key words
paeoniflorol/traumatic brain injury/nerve function/inflammation分类
医药卫生引用本文复制引用
姜乔继,杨红伟,丁亮,韦敏,万政强..基于p38 MAPK/NF-κB信号通路探究芍药醇对创伤性脑损伤小鼠神经保护作用的机制研究[J].临床神经外科杂志,2024,21(4):371-376,6.基金项目
江苏省医学创新中心建设项目(CXZX202212) （CXZX202212）
江苏省研究生科研与实践创新计划课题项目(SJCX23_1405) （SJCX23_1405）
