环境与职业医学2024，Vol.41Issue(7)：807-813,7.DOI:10.11836/JEOM24058
维生素C对染Cr(Ⅵ)小鼠肠道菌群紊乱的影响
Effect of vitamin C on intestinal flora disorders in Cr(Ⅵ)-contaminated mice
摘要
Abstract
[Background]Hexavalent chromium[Cr(Ⅵ)]exposure can cause structural disruption of intestinal flora and functional impairment.Vitamin C(VC)is one of the essential micronutrients,which plays an important role in promoting the growth of intestinal probiotics,improving the intestinal barrier,and maintaining the homeostasis of intestinal flora.However,the regulatory effect of VC on the intestinal flora disorders caused by Cr(Ⅵ)exposure remains to be investigated. [Objective]To investigate the effect of VC on intestinal flora disruption in mice due to Cr(Ⅵ)exposure. [Methods]Thirty-two SPF-grade C57BL/6 mice were acclimatized and fed for 3 d and randomly divided into control(Con),VC,potassium dichromate[K2Cr2O7,Cr(Ⅵ)],and VC+K2Cr2O7[VC+Cr(Ⅵ)]groups.At 8:00 a.m.on day 4,the Con group(double-distilled water given by gavage and injected intraperitoneally),the VC group(VC given by gavage and double-distilled water injected intraperitoneally),the Cr(Ⅵ)group(double-distilled water given by gavage and K2Cr2O7 solution injected intraperitoneally),and the VC+Cr(Ⅵ)group(VC given by gavage and K2Cr2O7 solution injected intraperitoneally)were treated.The dose of VC was 200 mg·kg-1,and the dose of K2Cr2O7 was 1.25 mg·kg-1.The mice were treated for 45 consecutive days and then executed,the contents of the colon were sampled in sterile freezing tubes,and three replicates were collected from each group.After labeling,the samples were immediately put into liquid nitrogen for rapid freezing.After all the samples were collected,they were transferred to a-80℃ultra-low temperature refrigerator for storage.Samples of colon contents were analyzed for intestinal flora structure by high-throughput sequencing and bioinformatics software. [Results]The Cr(Ⅵ)exposure resulted in reduced body weight gain values in mice compared to the Con group.Pathological changes oc-curred in the ileal tissue of mice,with significant inflammatory cell infiltration in the Cr(Ⅵ)group and reduced inflammatory cell infiltration in the VC+Cr(Ⅵ)group.The number of operational taxonomic units(OTUs)of intestinal flora was altered in the Cr(Ⅵ)group of mice.In the α diversity analysis,the mean Sobs index in the Cr(Ⅵ)group was 240.333±67.796,the Chao index was 258.173±64.813,and the Ace index was 259.481±66.891,which were significantly lower than those in the Con group(P＜0.05),the PD whole tree index in the Cr(Ⅵ)group was 27.863±2.399,which was significantly higher than that in the Con group(P＜0.05),and the VC intervention significantly reversed the changes of the above indexes due to Cr(Ⅵ)exposure(P＜0.05).In the β diversity analysis,the principal coordinates analysis(PCoA)results showed a significant separation between the Cr(Ⅵ)group and the Con group,and after the VC intervention,there was a retraction of the separation trend and the difference was reduced.The multi-sample similarity dendrogram results showed that the control and the VC groups clustered together first,then with the VC+Cr(Ⅵ)group,and finally with the Cr(Ⅵ)group.The abundances of Bacteroidetes,Saccharibacteria,and Tenericutes in the intestine of mice in the Cr(Ⅵ)group were decreased,and the abundance of Firmicutes was in-creased;the abundances of Lactobacillus,Alistipes,Bacteroides,and Ruminiclostridium were also increased.Included among these,Bac-teroides showed a significantly higher abundance compared to the control mice(P＜0.05).Changes in the abundances of phyla and genera of the above mentioned gut microorganisms were reversed after the VC intervention. [Conclusion]Cr(Ⅵ)exposure can lead to intestinal damage and disorganization of the intestinal flora structure in mice,while VC intervention can ameliorate the above changes to a certain extent and normalize the intestinal flora structure.关键词
维生素C/六价铬/肠道菌群/多样性/相对丰度/高通量测序Key words
vitamin C/hexavalent chromium/intestinal flora/diversity/relative abundance/high-throughput sequencing分类
医药卫生引用本文复制引用
张丽敏,李晓红,刘晨,刘玉梅,吴学谦,舒明,周健,徐东群,王秦,李万伟..维生素C对染Cr(Ⅵ)小鼠肠道菌群紊乱的影响[J].环境与职业医学,2024,41(7):807-813,7.基金项目
山东省自然科学基金面上项目(ZR2020MH336) （ZR2020MH336）
山东省中医药科技项目(M-2023299) （M-2023299）
潍坊市科学技术发展计划项目(2022GX015) （2022GX015）
