上海针灸杂志2024，Vol.43Issue(8)：906-914,9.DOI:10.13460/j.issn.1005-0957.2024.08.0906
耳穴贴压联合电针对膝骨关节炎模型大鼠滑膜纤维化及内质网应激的抑制作用研究
Study of the inhibiting effects of ear point sticking plus electroacupuncture on synovial fibrosis and endoplasmic reticulum stress in knee osteoarthritis model rats
摘要
Abstract
Objective To observe the inhibiting effects of ear point sticking plus electroacupuncture on synovial fibrosis and endoplasmic reticulum stress in knee osteoarthritis model rats.Method Sixty Sprague-Dawley(SD)rats were randomized into a normal group(10 rats)and another group(50 rats).After successful modeling,rats in the another group were re-randomized into a model group,an ear point group,an electroacupuncture group,a combined group,and a medication group,each having 10 rats.Rats in the ear point group received magnetic pearl plasters applied to bilateral ear Kidney point(CO10),the electroacupuncture group received electroacupuncture intervention,and the medication group received Celecoxib solution via gavage.The rat knee joint swelling severity was observed in each group.The serum levels of interleukin(IL)-1,IL-6,tumor necrosis factor-α(TNF-α),manganese superoxide dismutase(Mn-SOD),catalase(CAT),and malondialdehyde(MDA)were detected.The levels of cyclooxygenase-2(COX-2),prostaglandin E2(PGE2),and nitric oxide(NO)in the joint fluid were determined.The IL-1β and TNF-α levels in the synovial tissues were also checked.The pathological changes in the cartilage tissues were observed,and the mRNA and protein expression levels of glucose regulated protein 78(GRP78),C/EBP-homologous protein(CHOP),matrix metalloproteinase 13(MMP-13),α-smooth muscle actin(α-SMA),tissue inhibitor of metallo-proteinase 1(TIMP1),collagen I(Col-Ⅰ),and collagen Ⅱ(Col-Ⅱ)in the synovial tissues were compared among the groups.Result Compared with the normal group,the rat joint swelling degree,the serum levels of IL-1,IL-6,TNF-α,and MDA,the levels of COX-2,PGE2,and NO in the joint fluid,and the IL-1β and TNF-α levels in the synovial tissues,as well as the mRNA and protein expression levels of GRP78,CHOP,MMP-13,α-SMA,TIMP1,Col-Ⅰ,and Col-Ⅱin the synovial tissues all increased significantly in the model group(P＜0.05);the serum levels of Mn-SOD and CAT dropped significantly in the model group(P＜0.05).Compared with the model group,the rat joint swelling degree,the serum levels of IL-1,IL-6,TNF-α,and MDA,the levels of COX-2,PGE2,and NO in the joint fluid,the IL-1β and TNF-α levels in the synovial tissues,and the mRNA and protein expression levels of GRP78,CHOP,MMP-13,α-SMA,TIMP1,Col-Ⅰ,and Col-Ⅱ in the synovial tissues all decreased significantly in the ear point,electroacupuncture,combined,and medication groups(P＜0.05);the serum levels of Mn-SOD and CAT increased significantly(P＜0.05);pathological changes in the articular cartilage tissues improved notably.Compared with the ear point and electroacupuncture groups,the joint swelling degree,the serum levels of IL-1,IL-6,TNF-α,and MDA,the levels of COX-2,PGE2,and NO in the joint fluid,the levels of IL-1β and TNF-α in the synovial tissues,and the mRNA and protein expression levels of GRP78,CHOP,MMP-13,α-SMA,TIMP1,Col-Ⅰ,and Col-Ⅱ in the synovial tissues all dropped significantly in the combined group(P＜0.05);the serum levels of Mn-SOD and CAT increased markedly(P＜0.05).Conclusion Ear point sticking plus electroacupuncture can improve joint swelling degree,inhibit pathological progression,mitigate inflammatory responses in the synovial tissues,and repair oxidative stress damage to repair synovial tissue fibrosis and endoplasmic reticulum stress responses in knee osteoarthritis rats.关键词
针刺疗法/电针/耳穴贴压/骨关节炎,膝/关节肿胀/炎症反应/内置网应激/大鼠Key words
Acupuncture therapy/Electroacupuncture/Ear point sticking/Osteoarthritis,Knee/Joint swelling/Inflammatory responses/Endoplasmic reticulum stress/Rats分类
医药卫生引用本文复制引用
樊远志,吴耀持,张峻峰,陈威,李艳..耳穴贴压联合电针对膝骨关节炎模型大鼠滑膜纤维化及内质网应激的抑制作用研究[J].上海针灸杂志,2024,43(8):906-914,9.基金项目
上海市中医药管理局基金项目(SHGZS-202219) （SHGZS-202219）
上海市卫生健康委员会、上海市中医药管理局项目[ZY(2021-2023)-0104-02-JS-12] （2021-2023）
上海中医药传承创新发展三年行动计划[ZY(2021-2023)-0205-04] （2021-2023）
