中国森林病虫2024，Vol.43Issue(3)：21-26,6.DOI:10.19688/j.cnki.issn1671-0886.20240010
松材线虫侵染对油松的生理生化影响
Effects of Bursaphelenchus xylophilus infestation on physiological and biochemical responses of Pinus tabuliformis
摘要
Abstract
To study the changes of some physiological and biochemical substances in the needles of Pinus tabuliformis after infestation with Bursaphelenchus xylophilus,the 5-year-old potted seedlings of P.tabuliformis were selected as the research objects.The activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),phenylalanine ammonialyase(PAL),contents of osmotic regulatory substances and secondary metabolites in P.tabuliformis needles were tested.The results showed that:the POD activity of P.tabuliformis needles was lower than that of the control group five days and 20 days after inoculation.The CAT activity was significantly higher than the control group 30 days after inoculation.SOD activity decreased slightly at first and then increased gradually,peaking at 30 days after inoculation and significantly higher than the control group.The PAL activity showed a trend of continuous increase,and reached a significant difference level compared with the control group 20 and 30 days after inoculation.The content of soluble protein initially increased and then decreased,peaking at 10 days after inoculation and was significantly higher than the control group at both 10 and 20 days after inoculation.The betaine content in the experimental group reached the peak value five days after inoculation,which was significantly higher than that in the control group.The flavonoid content increased initially and then decreased,being significantly higher than that in the control group 20 and 30 days after inoculation.Therefore,the activity of various defense enzymes and physiological and biochemical substances in P.tabuliformis seedlings would change dynamically when infected by B.xylophilus,and induce P.tabuliformis to produce defense response.关键词
松材线虫/油松/抗氧化酶/生理生化/次生代谢产物Key words
Bursaphelenchus xylophilus/Pinus tabuliformis/antioxidant enzyme/physiology and biochemistry/secondary metabolite分类
农业科技引用本文复制引用
王建国,王建军,马静怡,姜旭,周志权,石峰..松材线虫侵染对油松的生理生化影响[J].中国森林病虫,2024,43(3):21-26,6.基金项目
辽宁省自然科学基金项目"松材线虫在东北地区的传播媒介和控制技术"(2021-MS-053) （2021-MS-053）
沈阳市农村科技特派团专项"康平县沙金台乡和西关屯乡林业病虫害防治科技特派团"(23-411-2-18) （23-411-2-18）
国家自然科学基金项目"油松林下血红铆钉菇的生态位及其对子实体发生的影响机制"(32071674) （32071674）
