现代科学仪器2024，Vol.41Issue(4)：36-42,7.
人参PR13基因克隆及其蛋白的生物信息学分析
Cloning of the PR13 gene of Panax ginseng and bioinformatic analysis of proteins
摘要
Abstract
To predict the structures and functions of PR13 by Method using MEGA X,Expasy-ProtParam,ExPASy-ProtScale,SignaIP-5.0,TMHMM Servery 2.0,InterPro,STRING,SOPMA,Cell-PLoc 2.0,Discovery Studio 2019 Client.Result The result showed that the molecular weight of PR13 was 8213.73 Da,furthermore,PR13 was identified as a hydrophilic basic protein which contained signal peptides,but without transmembrane structures.About secondary structure,it contained many structures of α-helices(38.67%)and irregular coils(44.00%),and some of β-Fold(6.67%)Extend Chain(10.67%).Following Gene Ontology(GO)annotation,PR13 is a killing of cells of another organism,defense to fungus or others and extracellular region.And the three-dimensional structure of proteins was drawn.Conclusion The study provides a theoretical basis for further study on mechanism of PR proteins and breeding of Panax ginseng.关键词
病程相关蛋白/系统发育树/生物信息学分析Key words
Pathogenesis related protein/Phylogenetic tree/Bioinformatics analysis引用本文复制引用
孙天霞,张勋,吴剑,杨子奇,杨晓峰,宋远航,刘志美,张淼,邹明强,惠歌..人参PR13基因克隆及其蛋白的生物信息学分析[J].现代科学仪器,2024,41(4):36-42,7.基金项目
吉林省科技发展计划项目(20240304117SF) （20240304117SF）
长春海关科技项目(CCHG2023KY04)中国海关科学技术研究中心研课(2023HX07) （CCHG2023KY04）
人参抗病基因筛选及活性研究(YDZJ202301ZYTS204) （YDZJ202301ZYTS204）
基于TLR4/NLRP3信号通路探讨豁痰解毒通络方干预冠状动脉粥样硬化的作用及机制(2024031). （2024031）
