医药导报2024，Vol.43Issue(9)：1387-1392,6.DOI:10.3870/j.issn.1004-0781.2024.09.005
基于"敲除策略"筛选高良姜乙酸乙酯部位抗幽门螺杆菌胃炎活性成分
Screening of Anti-Helicobacter pylori Gastritis Active Component of the Ethyl Acetate Extract of Alpinia officinarum Hance Based on the Knockout Strategy
摘要
Abstract
Objective To screen the anti-Helicobacter pylori gastritis active components of the ethyl acetate extract of Alpinia officinarum Hance.Methods The"knock-out"strategy combined with high-performance liquid chromatography(HPLC)detection was developed to separate the components of the ethyl acetate extract of A.officinarum while obtaining the negative samples without the components.A human gastric epithelial cell(GES-1)model of H.pylori gastritis was established,and the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),interleukin-8(IL-8)and interleukin-1β(IL-1β)in the supernatant of the cells were determined by enzyme-linked immunosorbent assay(ELISA).Results The total flavonoid fraction,the negative fraction without total diphenylheptanoids,the negative fraction without 5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone(DHPA),and galangin significantly reduced IL-6 levels in the supernatant of H.pylori infected GES-1 cells at a concentration of 8 μg·mL-1 with 24 h incubation.The total flavonoid fraction strongly inhibited the release of IL-6,TNF-α,IL-8,and IL-1β from H.pylori gastritis GES-1 cells at a concentration of 16 μg·mL-1.Conclusions The total flavonoid fraction is the major anti-H.pylori gastritis active component of the ethyl acetate extract of A.officinarum.The results lay the foundation for further elucidation of the material basis of A.officnarum against H.pylori gastritis.关键词
高良姜/幽门螺杆菌胃炎/敲除/活性组分/活性成分Key words
Alpinia officinarum Hance/H.pylori gastritis/Knock out/Active component/Active ingredient分类
中医学引用本文复制引用
罗鑫,郑吴殷晓,詹健婷,杨敬宇,马浩然,干国平,尤朋涛,叶晓川,刘丹..基于"敲除策略"筛选高良姜乙酸乙酯部位抗幽门螺杆菌胃炎活性成分[J].医药导报,2024,43(9):1387-1392,6.基金项目
国家自然科学基金资助项目(82173960、31400302). （82173960、31400302）
