针刺研究2024，Vol.49Issue(8)：797-804,8.DOI:10.13702/j.1000-0607.20230860
艾灸对膝关节骨关节炎大鼠血浆氨基酸代谢的影响
Effect of moxibustion on plasma amino acid metabolism in rats with knee osteoarthritis
摘要
Abstract
Objective To observe the effect of moxibustion at"Zusanli"(ST36)on the plasma amino acid metabolism in rats with knee osteoarthritis(KOA),and to explore the amino acid metabolism mechanism of moxibustion in repairing cartilage injury in KOA.Methods A total of 30 SD rats were randomly divided into normal,model and moxibustion groups,with 10 rats in each group.Rats in the model and moxibustion groups were injected with the mixture of L-cysteine and papain into bilateral knee joint cavity to make the KOA model.The moxibustion group received moxibustion at bilateral ST36 for 30 min,once daily for 30 days.At the end of the experiment,the swelling degree of knee joint was calculated,the mechanical pain threshold was measured by the Von Frey filament,the cartilage tissue injury was observed by HE staining,the matrix metalloproteinase-13(MMP-13)content in the synovial tissue was detected by enzyme-linked immunosorbent assay(ELISA),and the differential amino acid metabolites in plasma were detected and screened by liquid chromatography-mass spectrometry(LC-MS).Results Compared with the normal group,the model group showed irregular cartilage surface,decreased number of chondrocytes,uneven distribution,and local clusters of chondrocytes;the contour of the tide line was blurred.The degree of joint swelling in the model group was higher than that in the normal group(P＜0.01),the mechanical pain threshold was lower(P＜0.01),and the content of MMP-13 in synovial tissue was higher(P＜0.01).The contents of proline and tryptophan in the model group were down-regulated(P＜0.01,P＜0.05).Compared with the model group,the cartilage tissue damage and knee joint swelling were decreased(P＜0.05),mechanical pain threshold was increased(P＜0.05),MMP-13 content in synovial tissue and levels of glutamate and histidine expression were decreased(P＜0.01,P＜0.05).Conclusion Moxibustion at ST36 significantly alleviated arthritis-related swelling and pain in KOA model rats,attenuated cartilage damage,and regulated levels of certain plasma amino acid metabolites.Moxibustion may regulate KOA cartilage synthesis and degradation through amino acid metabolic pathways such as proline,tryptophan,glutamate and histidine,exerting anti-inflammatory,analgesic,and protection of cartilage injury effects.关键词
艾灸/膝关节骨关节炎/氨基酸代谢/软骨损伤Key words
Moxibustion/Knee osteoarthritis/Amino acid metabolism/Cartilage injury引用本文复制引用
魏霞,刘磊,段文秀,张帆,舒琪,王茜伊,吴子建,蔡荣林,胡玲..艾灸对膝关节骨关节炎大鼠血浆氨基酸代谢的影响[J].针刺研究,2024,49(8):797-804,8.基金项目
国家自然科学基金项目(No.82105020) （No.82105020）
国家"973计划"项目(No.2015CB554504) （No.2015CB554504）
安徽省自然科学基金项目(No.2108085QH363、2108085MH307) （No.2108085QH363、2108085MH307）
安徽中医药大学人才支持项目(No.2020rcyb003) （No.2020rcyb003）
