麦粒灸调控TLR4/MyD88/NF-κB信号通路减轻溃疡性结肠炎小鼠结肠损伤的机制研究OA北大核心CSTPCDMEDLINE

Objective To observe the effect of moxibustion with seed-size moxa cones on the Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear transcription factor-KB(NF-KB)signaling pathway in mice with ulcerative colitis(UC),so as to explore the therapeutic mechanism of moxibustion with seed-size moxa cones on colonic injury in UC.Methods Forty male C57BL/6 mice were randomly divided into blank group,model group,moxibustion group,and western medicine group,with 10 mice in each group.The UC mouse model was established by 3％DSS solution by free drinking for 7 consecutive days.Mice in the moxibustion group were treated with seed-size moxa cones at"Zhongwan"(CV12),"Tianshu"(ST25)and"Shangjuxu"(ST37),3 moxa cones per point,with each cone applied for approximately 30 s,while mice in the western medicine group were orally administered with 300 mg/kg mesalazine solution,which were both conducted once a day for 7 consecutive days.The general condition of mice was observed every 2 days,and the disease activity index(DAI)score was calculated.HE staining was used to observe the morphology of colonic tissue in mice.ELISA was used to detect the serum interleukin(IL)-1 β,tumor necrosis factor(TNF)-α,IL-6,and IL-8 contents.Immunohistochemistry was used to detect the positive expression of TLR4 and MyD88 in colonic tissue of mice.Real-time fluorescence quantitative PCR was used to detect the expression levels of TLR4,MyD88,and NF-κB p65 mRNAs in colonic tissue.Results Compared with the blank group,varying degrees of soft or watery stools were observed,colon length and body weight were decreased(P＜0.01)in mice of the model group,while DAI score,colon weight index,mucosal damage score,colonic pathological score,serum IL-1β,TNF-α,IL-6,and IL-8 contents,positive expressions of TLR4 and MyD88,and TLR4,MyD88,and NF-κB p65 mRNA expressions in colonic tissue were increased(P＜0.01).Compared with the model group,improved fecal characteristics were observed,colon length and body weight were increased(P＜0.01)in mice of the moxibustion group and western medicine group,while DAI scores,colon weight indexes,mucosal damage scores,colonic pathological score,serum contents of IL-1 β,TNF-α,IL-6,and IL-8,positive expressions of TLR4 and MyD88,and TLR4,MyD88,and NF-κB p65 mRNA expressions in colonic tissue were decreased(P＜0.01,P＜0.05).There was no significant difference in the above indicators between the moxibustion group and the western medicine group.Conclusion Moxibustion with seed-size moxa cones may alleviate colonic injury in UC mice by regulating the TLR4/MyD88/NF-κB signaling pathway and reducing the release of inflammatory factors.